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Mouse S100A8 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB3059V Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3059V-100UG

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Mouse S100A8 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 40% cross-reactivity with recombinant human (rh) S100A8 is observed and no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A9, rmS100A10, rmS100A11, rh
Source
Monoclonal Rat IgG2b Clone # 335806
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant mouse S100A8
Met1-Glu89 (predicted)
Accession # P27005
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: S100A8

Mouse S100A8 (also CP-10, calgranulin A and MRP8) is a 10 kDa member of the S100 family of calcium-binding proteins. The S100A8 protein is 89 amino acids (aa) in length and contains short, sequential modules. There is an N-terminal alpha -helix, followed by a calcium-binding EF-hand segment, and a C-terminal alpha -helix. S100A8 will noncovalently heterodimerize with S100A9. In the presence of calcium, this heterodimer will form a heterotetramer. The dimeric complex is both intracellular and extracellular. It binds heparan sulfate and is chemotactic for PMNs and macrophages. Mouse S100A8 shares 80% and 57% aa sequence identity with rat and human S100A8, respectively.

Long Name
S100 Calcium Binding Protein A8
Entrez Gene IDs
6279 (Human); 20201 (Mouse)
Alternate Names
60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100A8; Urinary stone protein band A

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