Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 40% cross-reactivity with recombinant human (rh) S100A8 is observed and no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A9, rmS100A10, rmS100A11, rhS100B, or rhS100P is observed.
Monoclonal Rat IgG2B Clone # 335806
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse S100A8 Met1-Glu89 (predicted) Accession # P27005
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
S100A8 in NIH-3T3 Mouse Cell Line. S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10 µg/mL Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of S100A8 Rat by Western Blot. Western blot shows lysates of mouse leukocyte. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse S100A8 (also CP-10, calgranulin A and MRP8) is a 10 kDa member of the S100 family of calcium-binding proteins. The S100A8 protein is 89 amino acids (aa) in length and contains short, sequential modules. There is an N-terminal alpha -helix, followed by a calcium-binding EF-hand segment, and a C-terminal alpha -helix. S100A8 will noncovalently heterodimerize with S100A9. In the presence of calcium, this heterodimer will form a heterotetramer. The dimeric complex is both intracellular and extracellular. It binds heparan sulfate and is chemotactic for PMNs and macrophages. Mouse S100A8 shares 80% and 57% aa sequence identity with rat and human S100A8, respectively.
S100 Calcium Binding Protein A8
Entrez Gene IDs:
6279 (Human); 20201 (Mouse)
60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100A8; Urinary stone protein band A
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