Mouse S100A8 Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 40% cross-reactivity with recombinant human (rh) S100A8 is observed and no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A9, rmS100A10, rmS100A11, rhS100B, or rhS100P is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 335806
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse S100A8
    Met1-Glu89 (predicted)
    Accession # P27005
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
S100A8 in NIH-3T3 Mouse Cell Line. S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10 µg/mL Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of S100A8 Rat by Western Blot. Western blot shows lysates of mouse leukocyte. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A8

Mouse S100A8 (also CP-10, calgranulin A and MRP8) is a 10 kDa member of the S100 family of calcium-binding proteins. The S100A8 protein is 89 amino acids (aa) in length and contains short, sequential modules. There is an N-terminal alpha -helix, followed by a calcium-binding EF-hand segment, and a C-terminal alpha -helix. S100A8 will noncovalently heterodimerize with S100A9. In the presence of calcium, this heterodimer will form a heterotetramer. The dimeric complex is both intracellular and extracellular. It binds heparan sulfate and is chemotactic for PMNs and macrophages. Mouse S100A8 shares 80% and 57% aa sequence identity with rat and human S100A8, respectively.

  • Long Name:
    S100 Calcium Binding Protein A8
  • Entrez Gene IDs:
    6279 (Human); 20201 (Mouse)
  • Alternate Names:
    60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100A8; Urinary stone protein band A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development
    Authors: N Makthal, K Nguyen, H Do, M Gavagan, P Chandrangs, JD Helmann, RJ Olsen, M Kumaraswam
    EBioMedicine, 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. The calcium-binding protein complex S100A8/A9 has a crucial role in controlling macrophage-mediated renal repair following ischemia/reperfusion.
    Authors: Dessing, Mark C, Tammaro, Alessand, Pulskens, Wilco P, Teske, Gwendoli, Butter, Loes M, Claessen, Nike, van Eijk, Marco, van der Poll, Tom, Vogl, Thomas, Roth, Johannes, Florquin, Sandrine, Leemans, Jaklien
    Kidney Int, 2015;87(1):85-94.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Visualized macrophage dynamics and significance of S100A8 in obese fat.
    Authors: Sekimoto R, Fukuda S, Maeda N, Tsushima Y, Matsuda K, Mori T, Nakatsuji H, Nishizawa H, Kishida K, Kikuta J, Maijima Y, Funahashi T, Ishii M, Shimomura I
    Proc Natl Acad Sci U S A, 2015;112(16):E2058-66.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: in vitro
  4. The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway.
    Authors: Yano, Junko, Kolls, Jay K, Happel, Kyle I, Wormley, Floyd, Wozniak, Karen L, Fidel, Paul L J
    PLoS ONE, 2012;7(9):e46311.
    Species: Mouse
    Sample Type: Vaginal Lavage
    Application: ELISA Development
  5. S100A8/A9 is not involved in host defense against murine urinary tract infection.
    Authors: Dessing MC, Butter LM, Teske GJ
    PLoS ONE, 2010;5(10):e13394.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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