Mouse Semaphorin 4B Alexa Fluor® 647-conjugated Antibody
Mouse Semaphorin 4B Alexa Fluor® 647-conjugated Antibody Summary
Accession # NP_038687
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Semaphorin 4B in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4B and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4B and eGFP was stained with either (A) Rat Anti-Mouse Semaphorin 4B Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB6239R) or (B) Rat IgG2A Alexa Fluor 647 Isotype Control (Catalog # IC006R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 4B
Semaphorin 4B (Sema4B; also SemaC) is a 95‑100 kDa, class IV member of the semaphorin family of proteins. It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses. Mature mouse Sema4B is a type I transmembrane glycoprotein that is 793 amino acids (aa) in length. It contains a 673 aa extracellular region (aa 31‑703) that is characterized by one Sema domain (aa 57‑492), a PSI region (aa 512‑582), and an Ig‑like C2‑type domain (aa 589‑649). There are three phosphoserines in the cytoplasmic tail. Over aa 31‑703, mouse Sema4B shares 86% and 95% aa identity with human and rat Sema4B, respectively.
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