Mouse Semaphorin 4B Alexa Fluor® 647-conjugated Antibody

Discontinued Product

FAB6239R has been discontinued.
Detection of Semaphorin 4B in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4B and eGFP by Flow Cytometry.
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Mouse Semaphorin 4B Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Semaphorin 4B in direct ELISAs. Stains mouse Semaphorin 4B transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Rat IgG2A Clone # 761915
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4B
Leu31-Glu703
Accession # NP_038687
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Semaphorin 4B in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4B and eGFP by Flow Cytometry. View Larger

Detection of Semaphorin 4B in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4B and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4B and eGFP was stained with either (A) Rat Anti-Mouse Semaphorin 4B Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB6239R) or (B) Rat IgG2A Alexa Fluor 647 Isotype Control (Catalog # IC006R). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Semaphorin 4B

Semaphorin 4B (Sema4B; also SemaC) is a 95‑100 kDa, class IV member of the semaphorin family of proteins. It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses. Mature mouse Sema4B is a type I transmembrane glycoprotein that is 793 amino acids (aa) in length. It contains a 673 aa extracellular region (aa 31‑703) that is characterized by one Sema domain (aa 57‑492), a PSI region (aa 512‑582), and an Ig‑like C2‑type domain (aa 589‑649). There are three phosphoserines in the cytoplasmic tail. Over aa 31‑703, mouse Sema4B shares 86% and 95% aa identity with human and rat Sema4B, respectively.

Entrez Gene IDs
10509 (Human); 20352 (Mouse); 293042 (Rat)
Alternate Names
KIAA1745sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4B; MGC131831; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4B; SEMA4B; SEMAC; Semaphorin 4B; semaphorin-4B; SemC

Product Datasheets

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