Detects mouse Semaphorin 4F in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Semaphorin 4B, rmSemaphorin 4C, and rmSemaphorin 4D is observed.
Detection of Mouse Semaphorin 4F by Western Blot. Western blot shows recombinant mouse Semaphorin 4F. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Semaphorin 4F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7200) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for recombinant mouse Semaphorin 4F at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 4F
Semaphorin 4F (Sema4F; also Sema W) is a 90-100 kDa, class IV member of the semaphorin family of proteins. It is expressed by select cell types, including Schwann cells and neurons, and likely acts as an inhibitor of cell proliferation by Schwann cells, and a regulator of postsynaptic glutamatergic synapses on neuron dendrites. Mature mouse Sema4F is a type I transmembrane protein that is 737 amino acids (aa) in length. It contains a 627 aa extracellular region (aa 41-667) that is characterized by the presence of one Sema domain (aa 71-493), a PSI region (aa 518-569), and a C2-type Ig-like domain (aa 586-641). Over aa 41-667, mouse Sema4F shares 92% and 98% aa identity with human and rat Sema4F, respectively.
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