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Mouse Semaphorin 4F Antibody

Catalog #: AF7200 Datasheet / COA / SDS

Discontinued Product

AF7200 has been discontinued.
View all Semaphorin 4F products.
Detection of Mouse Semaphorin 4F by Western Blot.
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Mouse Semaphorin 4F Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Semaphorin 4F in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Semaphorin 4B, rmSemaphorin 4C, and rmSemaphorin 4D is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4F
Arg41-Gly667
Accession # Q9Z123
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Semaphorin 4F antibody by Western Blot. View Larger

Detection of Mouse Semaphorin 4F by Western Blot. Western blot shows recombinant mouse Semaphorin 4F. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Semaphorin 4F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7200) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for recombinant mouse Semaphorin 4F at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4F

Semaphorin 4F (Sema4F; also Sema W) is a 90-100 kDa, class IV member of the semaphorin family of proteins. It is expressed by select cell types, including Schwann cells and neurons, and likely acts as an inhibitor of cell proliferation by Schwann cells, and a regulator of postsynaptic glutamatergic synapses on neuron dendrites. Mature mouse Sema4F is a type I transmembrane protein that is 737 amino acids (aa) in length. It contains a 627 aa extracellular region (aa 41-667) that is characterized by the presence of one Sema domain (aa 71-493), a PSI region (aa 518-569), and a C2-type Ig-like domain (aa 586-641). Over aa 41-667, mouse Sema4F shares 92% and 98% aa identity with human and rat Sema4F, respectively.

               

Entrez Gene IDs
10505 (Human); 20355 (Mouse); 29745 (Rat)
Alternate Names
M-SEMA; m-Sema-M; PRO2353; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4F; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4F; Sema M; Sema W; SEMA4F; SEMAM; Semaphorin 4F; semaphorin-4F; semaphorin-M; semaphorin-W; SEMAW; SEMAWsemaphorin M

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