Mouse Siglec-1/CD169 PE-conjugated Antibody

  (2 citations)     
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Citations (2)
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Siglec-1/CD169 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Siglec‑1/CD169, -5, -6, -7, -8, -9, -10, -11, -14, recombinant mouse Siglec-2, -3, -E, -F, -G, or -H is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 645608
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Siglec-1/CD169
    Thr20-Leu1639 (predicted)
    Accession # Q62230
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example

Detection of Siglec‑1/CD169 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Siglec‑1/CD169 PE‑conjugated Monoclonal Antibody (Catalog # FAB5610P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Siglec-1/CD169

Siglec-1, also known as Sialoadhesin or CD169, is a 175-185 kDa type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.

  • Long Name:
    Sialic Acid Binding Ig-like Lectin 1
  • Entrez Gene IDs:
    6614 (Human); 20612 (Mouse); 311426 (Rat)
  • Alternate Names:
    C19orf75; CD169; Siglec1; Siglec-1; SIGLECL1 SIGLEC family like 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells
    Nat Commun, 2016;7(0):12719.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Induction of Siglec-1 by Endotoxin Tolerance Suppresses the Innate Immune Response by Promoting TGF-?1 Production
    Authors: Y Wu, C Lan, D Ren, GY Chen
    J. Biol. Chem., 2016;291(23):12370-82.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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