Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 750620
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Siglec-E
Gln19-Phe354
Accession # Q91Y57

Specificity

Detects mouse Siglec-E in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Siglec-3/CD33 or rmSiglec-F is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Siglec‑E APC‑conjugated Antibody

Detection of Siglec-E antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of Siglec‑E in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD11c Alexa Fluor® 488-conjugated Monoclonal Antibody (FAB69501G) and either (A) Rat Anti-Mouse Siglec-E APC-conjugated Monoclonal Antibody (Catalog # FAB5806A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Siglec‑E APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-E

Mouse Siglec-E (Salic acid binding Ig-like lectin E), also known as Siglecl1, is a 52 kDa (predicted) member of the CD33-related subfamily of Siglecs.

Long Name

Sialic Acid Binding Ig-like Lectin E

Alternate Names

SiglecE

Entrez Gene IDs

83382 (Mouse)

Gene Symbol

SIGLECE

UniProt

Additional Siglec-E Products

Product Documents for Mouse Siglec‑E APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Siglec‑E APC‑conjugated Antibody

For research use only

Citations for Mouse Siglec‑E APC‑conjugated Antibody

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FAQs

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