Mouse Siglec-E APC-conjugated Antibody

Catalog #: FAB5806A Datasheet / COA / SDS

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FAB5806A

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Detection of Siglec‑E in Mouse Splenocytes by Flow Cytometry.

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Mouse Siglec-E APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Siglec-E in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Siglec-3/CD33 or rmSiglec-F is observed.

Source

Monoclonal Rat IgG_2B Clone # 750620

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Siglec-E
Gln19-Phe354
Accession # Q91Y57

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Siglec‑E in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD11c Alexa Fluor® 488-conjugated Monoclonal Antibody (FAB69501G) and either (A) Rat Anti-Mouse Siglec-E APC-conjugated Monoclonal Antibody (Catalog # FAB5806A) or (B) Rat IgG_2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-E

Mouse Siglec-E (Salic acid binding Ig-like lectin E), also known as Siglecl1, is a 52 kDa (predicted) member of the CD33-related subfamily of Siglecs.

Long Name

Sialic Acid Binding Ig-like Lectin E

Entrez Gene IDs

83382 (Mouse)

Alternate Names

CD170;CD33L2;OBBP2;OB-BP2;Sialic acid-binding Ig-like lectin 5;SIGLEC5;SIGLEC-5; SiglecE; Siglec-E