Mouse Siglec-E APC-conjugated Antibody

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Detection of Siglec‑E in Mouse Splenocytes by Flow Cytometry.
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Mouse Siglec-E APC-conjugated Antibody Summary

Species Reactivity
Detects mouse Siglec-E in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Siglec-3/CD33 or rmSiglec-F is observed.
Monoclonal Rat IgG2B Clone # 750620
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse Siglec-E
Accession # Q91Y57
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Siglec-E antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Siglec‑E in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD11c Alexa Fluor® 488-conjugated Monoclonal Antibody (FAB69501G) and either (A) Rat Anti-Mouse Siglec-E APC-conjugated Monoclonal Antibody (Catalog # FAB5806A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-E

Mouse Siglec-E (Salic acid binding Ig-like lectin E), also known as Siglecl1, is a 52 kDa (predicted) member of the CD33-related subfamily of Siglecs.

Long Name
Sialic Acid Binding Ig-like Lectin E
Entrez Gene IDs
83382 (Mouse)
Alternate Names
CD170;CD33L2;OBBP2;OB-BP2;Sialic acid-binding Ig-like lectin 5;SIGLEC5;SIGLEC-5; SiglecE; Siglec-E

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