Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 750620
View all Siglec-E Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Siglec-E
Gln19-Phe354
Accession # Q91Y57

Specificity

Detects mouse Siglec-E in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Siglec-3/CD33 or rmSiglec-F is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Siglec‑E Antibody

Detection of Mouse Siglec-E antibody in Mouse Splenocytes.

Detection of Mouse Siglec-E in Mouse Splenocytes.

Mouse Splenocytes were stained with Anti-Mouse Integrin aX/CD11c Phycoerythrin-conjugated Antibody and either (A) Rat IgG2B Isotype Control (Catalog # MAB0061) or (B) Rat Anti-Mouse Siglec-E Monoclonal Antibody (Catalog # MAB5806) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Applications for Mouse Siglec‑E Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Siglec-E

Mouse Siglec-E (Salic acid binding Ig-like lectin E), also known as Siglecl1, is a 52 kDa (predicted) member of the CD33-related subfamily of Siglecs.

Long Name

Sialic Acid Binding Ig-like Lectin E

Alternate Names

SiglecE

Entrez Gene IDs

83382 (Mouse)

Gene Symbol

SIGLECE

UniProt

Q91Y57

Additional Siglec-E Products

Product Documents for Mouse Siglec‑E Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Siglec‑E Antibody

For research use only

Citations for Mouse Siglec‑E Antibody

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