Detection of CD11c in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse MHC class II (I‑A/I‑E) APC‑conjugated Monoclonal Antibody (Catalog # FAB6118A) and either (A) Hamster Anti-Mouse CD11c Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB69501G) or (B) Hamster IgG Alexa Fluor 488 Control. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
The Integrin alpha X subunit, also known as CD11c, is a 150 kDa type I transmembrane protein that noncovalently heterodimerizes with the beta 2 subunit (CD18) to form alpha X/ beta 2, also known as p150/p95 and complement receptor type 4 (CR4). Integrin alpha X beta 2 is expressed on macrophages, dendritic cells, CD8+ T cells, and other leukocyte subsets. The 1097 aa mouse alpha X extracellular domain shares 71% and 87% amino acid (aa) identity with human and rat alpha X, respectively. One potential alpha X isoform is truncated at aa 828. Some adhesion partners of alpha X beta 2 are shared with alpha M beta 2/CD11b:CD18 (complement iC3b, ICAMs, vWF and fibrinogen) while other adhesion partners (osteopontin, Thy-1, plasminogen, heparin) are unique. Unlike alpha M beta 2, alpha X beta 2 is not constitutively active. alpha X beta 2 adhesion mediates cell proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.