Mouse SIGNR1/CD209b Biotinylated Antibody
Mouse SIGNR1/CD209b Biotinylated Antibody Summary
Accession # Q8CJ91
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
SIGNR1 belongs to the family of C-type lectins that participate in innate immune responses by binding and clearing pathogens (1‑3). SIGNR1 is one of four mouse proteins, encoded by separate genes, with significant homology to DC-SIGN and DC-SIGNR (4). Mouse SIGNR1 consists of a 53 amino acid (aa) cytoplasmic domain, a 20 aa transmembrane segment, and a 252 aa extracellular domain that contains a juxtamembrane neck region and a carbohydrate recognition domain (CRD) (4, 5). SIGNR1 is a 50kDa type II protein that forms high molecular weight aggregates as has been shown for DC-SIGN and DC-SIGNR (6, 7). The mouse SIGNR1 mRNA is extensively spliced, giving rise to multiple isoforms in addition to variability in the 5’ and 3’ untranslated regions. The alpha isoform is known as SIGNR1. The beta and gamma isoforms lack the transmembrane segment, with the gamma isoform also showing a 21 aa insertion in the CRD. The δ isoform lacks almost the entire CRD (5). Functional differences between the isoforms have not been described. Within the CRD, mouse SIGNR1 shares 67%‑70% aa sequence identity with mouse DC-SIGN, SIGNR2, 3, and 4, and 64%‑68% aa sequence identity with human DC-SIGN, human L-SIGN, and rat DC-SIGN. The CRD binds mannose, GlcNAc, and fucose structures on some bacterial strains, mycobacteria, and yeast (8). SIGNR1 is expressed on macrophages found in the splenic marginal zone, lymph node medulla, and peritoneum (6, 9, 10). It plays a dominant role in the clearance of circulating S. pneumoniae (11‑13), enhances TLR4 signaling (14), and cooperates with Dectin-1 in the nonopsonic clearance of fungal pathogens (9, 15). SIGNR1 also binds mouse ICAM-2, human ICAM-2 and -3, and HIV gp120 (10).
- Marshall, A.S.J. and S. Gordon (2004) Eur. J. Immunol. 34:18.
- Cambi, A. and C.G. Figdor (2005) Curr. Opin. Immunol. 17:345.
- McGreal, E.P. et al. (2005) Curr. Opin. Immunol. 17:18.
- Park, C.G. et al. (2001) Int. Immunol. 13:1283.
- Parent, S.A. et al. (2002) Gene 293:33.
- Kang, Y.-S. et al. (2003) Int. Immunol. 15:177.
- Feinberg, H. et al. (2005) J. Biol. Chem. 280:1327.
- Galustian, C. et al. (2003) Int. Immunol. 16:853.
- Taylor, P.R. et al. (2004) J. Immunol. 172:1157.
- Geijtenbeek, T.B.H. et al. (2002) Blood 100:2908.
- Koppel, E.A. et al. (2005) Eur. J. Immunol. 35:2962.
- Lanoue, A. et al. (2004) J. Exp. Med. 200:1383.
- Kang, Y.-S. et al. (2004) Proc. Natl. Acad. Sci. 101:215.
- Nagaoka, K. et al. (2005) Int. Immunol. 17:827.
- Takahara, K. et al. (2003) Int. Immunol. 16:819.
Citation for Mouse SIGNR1/CD209b Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization
Authors: J Lian, AJ Ozga, CL Sokol, AD Luster
Cell Rep, 2020;31(8):107679.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Supplemental Cell Selection Products
Supplemental ELISA Products
Reviews for Mouse SIGNR1/CD209b Biotinylated Antibody
There are currently no reviews for this product. Be the first to review Mouse SIGNR1/CD209b Biotinylated Antibody and earn rewards!
Have you used Mouse SIGNR1/CD209b Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image