Mouse Spinesin Biotinylated Antibody

Catalog # Availability Size / Price Qty
Product Details
Supplemental Products

Mouse Spinesin Biotinylated Antibody Summary

Species Reactivity
Detects mouse Spinesin in Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant human Spinesin is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Spinesin
Accession # NP_109634
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Spinesin (Catalog # 1928-SE)
5-15 µg/mL
Perfusion fixed frozen sections of mouse spinal cord

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spinesin

Spinesin, encoded by the TMPRSS5 gene, is a new member of type II transmembrane serine proteases (TTSPs) (1). Mouse Spinesin contains the following structural domains: a short N-terminal cytoplasmic tail, a transmembrane domain, a stem region and a serine protease domain (2). The domain structure of Spinesin is common to other TTSPs, many of which have additional domains. The stem region of Spinesin contains a scavenger receptor-like domain. There could be 4 types of transcripts due to alternative splicing (3). Type 4 predicts 10 extra amino acids at the N-terminus as compared to type 3. The ectodomain corresponding to type 3 (residues 61‑445) or type 4 (residues 71‑455) was expressed and purified as a single chain pro-enzyme. By SDS-PAGE, the pro-enzyme migrates as multiple forms, possibly due to differential glycosylation. The pro-enzyme can be activated by trypsin treatment. The resulting enzyme is active and its activity is measured as described above. The activated enzyme is a disulfide bond-linked dimer.

  1. Shibata, K. et al. (2000) Genome Res. 10:1757.
  2. Yamaguchi, Y. et al. (2002) J. Biol. Chem. 277:6806.
  3. Watanable, Y. et al. (2004) Biochem. Biophys. Res. Commun. 324:333.
Entrez Gene IDs
80975 (Human); 80893 (Mouse)
Alternate Names
EC 3.4.21; EC 3.4.21.-; EC; MGC141886; MGC148044; Spinesin; TMPRSS5; transmembrane protease serine 5; transmembrane protease, serine 5

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Spinesin Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse Spinesin Biotinylated Antibody and earn rewards!

Have you used Mouse Spinesin Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review