Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all TCAM-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TCAM-1
Ala20-Gly483
Accession # NP_083743

Specificity

Detects mouse TCAM-1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse TCAM-1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse TCAM‑1 (Catalog # 3546-TC)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TCAM-1

Mouse TCAM-1 (Testicular cell adhesion molecule-1) is a 59 kDa (predicted) type I transmembrane protein that is a member of the intracellular adhesion molecule (ICAM) family belonging to the immunoglobulin (Ig) superfamily (1). It is synthesized from a 548 amino acid (aa) precursor that contains an 18 aa signal sequence, a 465 aa extracellular region, a 25 aa transmembrane region, and a short cytoplasmic tail of 40 aa. The extracellular region contains five Ig domains and six potential N‑linked glycosylation sites (1). Mouse TCAM‑1 is 89% identical to rat TCAM‑1. Expression of TCAM‑1 is detected in the embryo on day 17 and in adult testis, and correlates with the presence of pachytene spermatocytes and spermatids in mouse testis development (1).

References

  1. Skatani, S. et al. (2000) Genome 43:957.

Long Name

Testicular Cell Adhesion Molecule 1, TCAM-1P

Alternate Names

TCAM1

Entrez Gene IDs

146771 (Human); 75870 (Mouse); 59305 (Rat)

Gene Symbol

TCAM1P

UniProt

NP_083743

Additional TCAM-1 Products

Product Documents for Mouse TCAM-1 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TCAM-1 Biotinylated Antibody

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

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