Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TCAM-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TCAM-1
Ala18-Gly481
Accession # AAH50746

Specificity

Detects mouse TCAM-1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse TCAM‑1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse TCAM‑1 (Catalog # 3546-TC)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TCAM-1

Mouse TCAM-1 (Testicular cell adhesion molecule-1) is a 59 kDa (predicted) type I transmembrane protein that is a member of the intracellular adhesion molecule (ICAM) family belonging to the immunoglobulin (Ig) superfamily (1). It is synthesized from a 548 amino acid (aa) precursor that contains an 18 aa signal sequence, a 465 aa extracellular region, a 25 aa transmembrane region, and a short cytoplasmic tail of 40 aa. The extracellular region contains five Ig domains and six potential N‑linked glycosylation sites (1). Mouse TCAM‑1 is 89% identical to rat TCAM‑1. Expression of TCAM‑1 is detected in the embryo on day 17 and in adult testis, and correlates with the presence of pachytene spermatocytes and spermatids in mouse testis development (1).

References

  1. Skatani, S. et al. (2000) Genome 43:957.

Long Name

Testicular Cell Adhesion Molecule 1, TCAM-1P

Alternate Names

TCAM1

Entrez Gene IDs

146771 (Human); 75870 (Mouse); 59305 (Rat)

Gene Symbol

TCAM1P

UniProt

AAH50746

Additional TCAM-1 Products

Product Documents for Mouse TCAM‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse TCAM‑1 Antibody

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

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