Mouse TER-119 Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1125V-025
Detection of TER‑119 in Mouse Bone Marrow Cells by Flow Cytometry.
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Mouse TER-119 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, and adult peripheral blood.
Source
Monoclonal Rat IgG2B Clone # TER-119
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C57BL/6 mouse day-14 fetal liver cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TER‑119 in Mouse Bone Marrow Cells by Flow Cytometry. View Larger

Detection of TER‑119 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse CD45 APC‑conjugated Monoclonal Antibody (Catalog # FAB114A) and either (A) Rat Anti-Mouse TER‑119 Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB1125V) or (B) Rat IgG2B Alexa Fluor 405 Isotype Control (Catalog # IC013V). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TER-119

The monoclonal antibody TER-119 is isolated from a hybridoma generated using splenocytes from a rat subcutaneously injected with day 14 BALB/c fetal liver cells (1). The TER-119 monoclonal antibody reacts with erythroid cells from the early proerythroblast to mature erythrocyte stages of development (1). The 52 kDa ligand for TER-119 is associated with glycophorin A on erythrocytes (1). TER-119 antibodies are frequently used in combination with other lineage depletion antibodies to enrich for mouse hematopoietic stem cells (2, 3).

References
  1. Kina, T. et al. (2000) Br. J. Haematol. 109:280.
  2. Ikuta, K. et al. (1990) Cell 62:863.
  3. Osawa, M.Y. et al., (1996) Hematopoietic Stem Cells in Weir’s Handbook of Experimental Immunology, Vol. 2, 5th Edition. Herzenberg, L.A. et al. eds. Blackwell Science, Cambridge, MA. pp. 66.1-66.5
Entrez Gene IDs
104231 (Mouse)
Alternate Names
Ly76; Ly-76; TER119; TER-119

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