Mouse TER-119 Antibody

Catalog # Availability Size / Price Qty
MAB1125
MAB1125-SP
Product Details
Citations (8)
FAQs
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Mouse TER-119 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell 62:863). TER-119 Antigen is a molecule associated with cell-surface glycophorin A (Kina, T. et al. (2000) Br. J. Haematol. 109:280). The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte (Kina, T. et al. (2000) Br. J. Haematol. 109:280).
Source
Monoclonal Rat IgG2B Clone # TER-119
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C57BL/6 mouse day-14 fetal liver cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Kina, T. et al. (2000) Br. J. Haematol. 109:280
 
Flow Cytometry
0.25 µg/106 cells
Mouse bone marrow
Immunohistochemistry
8-25 µg/mL
Immersion fixed frozen sections of mouse embryonic liver (13 d.p.c.)
Immunoprecipitation
Kina, T. et al. (2000) Br. J. Haematol. 109:280
 
CyTOF-reported
Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TER-119

The TER-119 Antigen has been used as a marker for erythroid cells from the early proerythroblast to mature erythrocyte stages of development. The 52-kDa TER-119 Antigen is associated with Glycophorin A on erythrocytes.

Entrez Gene IDs
104231 (Mouse)
Alternate Names
Ly76; Ly-76; TER119; TER-119

Product Datasheets

Citations for Mouse TER-119 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes
    Authors: R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams
    Nat Commun, 2019;10(1):2817.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Endothelial EphB4 maintains vascular integrity and transport function in adult heart
    Authors: G Luxán, J Stewen, N Díaz, K Kato, SK Maney, A Aravamudha, F Berkenfeld, N Nagelmann, HC Drexler, D Zeuschner, C Faber, H Schillers, S Hermann, J Wiseman, JM Vaquerizas, ME Pitulescu, RH Adams
    Elife, 2019;8(0):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: IHC
  3. Placental labyrinth formation in mice requires endothelial FLRT2-UNC5B signaling
    Authors: I Tai-Nagara, Y Yoshikawa, N Numata, T Ando, K Okabe, Y Sugiura, M Ieda, N Takakura, O Nakagawa, B Zhou, K Okabayashi, M Suematsu, Y Kitagawa, M Bastmeyer, K Sato, R Klein, S Navankasat, DY Li, S Yamagishi, Y Kubota
    Development, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Pharmacologic or Genetic Targeting of Glutamine Synthetase Skews Macrophages toward an M1-like Phenotype and Inhibits Tumor Metastasis
    Authors: EM Palmieri, A Menga, R Martín-Pér, A Quinto, C Riera-Domi, G De Tullio, DC Hooper, WH Lamers, B Ghesquière, DW McVicar, A Guarini, M Mazzone, A Castegna
    Cell Rep, 2017;20(7):1654-1666.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Motor neurons control blood vessel patterning in the developing spinal cord
    Authors: P Himmels, I Paredes, H Adler, A Karakatsan, R Luck, HH Marti, O Ermakova, E Rempel, ET Stoeckli, C Ruiz de Al
    Nat Commun, 2017;8(0):14583.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Hydrogels with precisely controlled integrin activation dictate vascular patterning and�permeability
    Authors: S Li, LR Nih, H Bachman, P Fei, Y Li, E Nam, R Dimatteo, ST Carmichael, TH Barker, T Segura
    Nat Mater, 2017;16(9):953-961.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Blood flow controls bone vascular function and osteogenesis
    Nat Commun, 2016;7(0):13601.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Integrin beta1 controls VE-cadherin localization and blood vessel stability.
    Authors: Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams R
    Nat Commun, 2015;6(0):6429.

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Isotype Controls

Rat IgG2B Isotype Control

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