Mouse TER-119 Alexa Fluor® 700-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, and adult peripheral blood.
  • Source
    Monoclonal Rat IgG2B Clone # TER-119
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    C57BL/6 mouse day-14 fetal liver cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of TER‑119 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse CD45 PE‑conjugated Monoclonal Antibody (Catalog # FAB114P) and either (A) Rat Anti-Mouse TER‑119 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1125N) or (B) Rat IgG2B Alexa Fluor 700 Isotype Control (Catalog # IC013N). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TER-119
The monoclonal antibody TER-119 is isolated from a hybridoma generated using splenocytes from a rat subcutaneously injected with day 14 BALB/c fetal liver cells (1). The TER-119 monoclonal antibody reacts with erythroid cells from the early proerythroblast to mature erythrocyte stages of development (1). The 52 kDa ligand for TER-119 is associated with glycophorin A on erythrocytes (1). TER-119 antibodies are frequently used in combination with other lineage depletion antibodies to enrich for mouse hematopoietic stem cells (2, 3).
  • References:
    1. Kina, T. et al. (2000) Br. J. Haematol. 109:280.
    2. Ikuta, K. et al. (1990) Cell 62:863.
    3. Osawa, M.Y. et al., (1996) Hematopoietic Stem Cells in Weir’s Handbook of Experimental Immunology, Vol. 2, 5th Edition. Herzenberg, L.A. et al. eds. Blackwell Science, Cambridge, MA. pp. 66.1-66.5
  • Entrez Gene IDs:
    104231 (Mouse)
  • Alternate Names:
    Ly76; Ly-76; TER119
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 700-conjugated Antibody

Ctrl IC013N  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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