Detection of TER‑119 in Mouse Bone Marrow Cells by Flow Cytometry.
Mouse bone marrow cells were stained with Rat Anti-Mouse CD45 PE‑conjugated Monoclonal Antibody (Catalog # FAB114P) and either (A) Rat Anti-Mouse TER‑119 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1125N) or (B) Rat IgG2B Alexa Fluor 700 Isotype Control (Catalog # IC013N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
The monoclonal antibody TER-119 is isolated from a hybridoma generated using splenocytes from a rat subcutaneously injected with day 14 BALB/c fetal liver cells (1). The TER-119 monoclonal antibody reacts with erythroid cells from the early proerythroblast to mature erythrocyte stages of development (1). The 52 kDa ligand for TER-119 is associated with glycophorin A on erythrocytes (1). TER-119 antibodies are frequently used in combination with other lineage depletion antibodies to enrich for mouse hematopoietic stem cells (2, 3).
Kina, T. et al. (2000) Br. J. Haematol. 109:280.
Ikuta, K. et al. (1990) Cell 62:863.
Osawa, M.Y. et al., (1996) Hematopoietic Stem Cells in Weir’s Handbook of Experimental Immunology, Vol. 2, 5th Edition. Herzenberg, L.A. et al. eds. Blackwell Science, Cambridge, MA. pp. 66.1-66.5