Mouse TER-119 Biotinylated Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell 62:863). TER-119 Antigen is a molecule associated with cell-surface glycophorin A (Kina, T. et al. (2000) Br. J. Haematol. 109:280). The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte (Kina, T. et al. (2000) Br. J. Haematol. 109:280).
  • Source
    Monoclonal Rat IgG2B Clone # TER-119
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    C57BL/6 mouse day-14 fetal liver cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    Kina, T. et al. (2000) Br. J. Haematol. 109:280
  • Flow Cytometry
    0.25 µg/106 cells
    Mouse bone marrow cells
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunoprecipitation
    Kina, T. et al. (2000) Br. J. Haematol. 109:280
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
TER‑119 in Mouse Embryo. TER‑119 was detected in immersion fixed frozen sections of mouse embryo (E15) using Rat Anti-Mouse TER‑119 Biotinylated Monoclonal Antibody (Catalog # BAM1125) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter­stained with hematoxylin (blue). Specific staining was localized to red blood cell plasma membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TER-119
The TER-119 Antigen has been used as a marker for erythroid cells from the early proerythroblast to mature erythrocyte stages of development. The 52-kDa TER-119 Antigen is associated with Glycophorin A on erythrocytes.
  • Entrez Gene IDs:
    104231 (Mouse)
  • Alternate Names:
    Ly76; Ly-76; TER119; TER-119
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Hepcidin regulates ferroportin expression and intracellular iron homeostasis of erythroblasts.
    Authors: Zhang DL, Senecal T, Ghosh MC
    Blood, 2011;118(10):2868-77.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IP
ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Biotinylated Isotype Control

Ctrl IC013B
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-Allophycocyanin

Flow F0050 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Avidin-Fluorescein

Flow F0030 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

MagCellect Streptavidin Ferrofluid

MAG999 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

EvenCoat Streptavidin Coated Plates

ELISA CP004
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse TER-119 Biotinylated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information