Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. The deduced amino acid sequence of mouse THBD predicts a signal peptide (aa 1 to 16) and a mature chain (aa 17 to 577) that consists of the following domains: C-type lectin (aa 31 to 167), EGF-like (aa 240 to 280, aa 283 to 323, aa 324 to 362, aa 364 to 404, aa 405 to 439, and aa 440 to 480), transmembrane (aa 518 to 541) and cytoplasmic (aa 542 to 577) (1). The R&D Systems rmTHBD consists of aa 17 to 517, corresponding to the extracellular portion of the type I membrane protein. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (2‑4). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal amino acid residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (5). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (6).
Mouse Thrombomodulin/BDCA-3 Alexa Fluor® 750-conjugated Antibody
R&D Systems | Catalog # FAB3894S
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Leu17-Ser517
Accession # P15306
Specificity
Clonality
Host
Isotype
Applications for Mouse Thrombomodulin/BDCA-3 Alexa Fluor® 750-conjugated Antibody
Flow Cytometry
Sample: bEnd.3 mouse endothelioma cell line
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: Thrombomodulin/BDCA-3
References
- Dittman, W.A. and P.W. Majerus (1989) Nucleic Acids Res. 17:802.
- Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. 24:1374.
- Wu, K.K. et al. (2000) Ann Med. 32:73.
- Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
- Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
- Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.
Alternate Names
Gene Symbol
UniProt
Additional Thrombomodulin/BDCA-3 Products
Product Documents for Mouse Thrombomodulin/BDCA-3 Alexa Fluor® 750-conjugated Antibody
Certificate of Analysis
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Product Specific Notices for Mouse Thrombomodulin/BDCA-3 Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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