Mouse Thrombomodulin/BDCA-3 PE-conjugated Antibody

(1 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Thrombomodulin/BDCA-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Thrombomodulin is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 461714
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Thrombomodulin/BDCA-3
    Leu17-Ser517
    Accession # P15306
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Thrombomodulin/BDCA‑3 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Thrombomodulin/BDCA‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB3894P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Thrombomodulin/BDCA-3

Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. The deduced amino acid (aa) sequence of mouse THBD predicts a signal peptide (aa 1 to 16) and a mature chain (aa 17 to 577) that consists of the following domains: C-type lectin (aa 31 to 167), EGF-like (aa 240 to 280, aa 283 to 323, aa 324 to 362, aa 364 to 404, aa 405 to 439, and aa 440 to 480), transmembrane (aa 518 to 541) and cytoplasmic (aa 542 to 577) (1). Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (2‑4). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal amino acid residues from its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisms are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (5). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (6). Over aa 17-517, mouse Thrombomodulin shares 84% and 66% aa sequence identity with rat and human thrombomoduli, respectively.

  • References:
    1. Dittman, W.A. and P.W. Majerus (1989) Nucleic Acids Res. 17:802.
    2. Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. Biol. 24:1374.
    3. Wu, K.K. et al. (2000) Ann Med. 32:73.
    4. Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
    5. Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
    6. Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.
  • Entrez Gene IDs:
    7056 (Human); 21824 (Mouse); 83580 (Rat)
  • Alternate Names:
    BDCA3; BDCA-3; CD141 antigen; CD141; Fetomodulin; THBD; THRM; Thrombomodulin; TM
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Pharmacological targeting of the thrombomodulin-activated protein C pathway mitigates radiation toxicity.
    Authors: Geiger H, Pawar SA, Kerschen EJ, Nattamai KJ, Hernandez I, Liang HP, Fernandez JA, Cancelas JA, Ryan MA, Kustikova O, Schambach A, Fu Q, Wang J, Fink LM, Petersen KU, Zhou D, Griffin JH, Baum C, Weiler H, Hauer-Jensen M
    Nat. Med., 2012;18(0):1123.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Mouse Thrombomodulin/BDCA-3 PE-conjugated Antibody FAB3894P
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Flow Cytometry Mouse Thrombomodulin/BDCA-3 PE-conjugated Antibody FAB3894P
Flow Cytometry: Mouse Thrombomodulin/BDCA-3 PE-conjugated Antibody [FAB3894P]

Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)
SpeciesMouse

Other Experimental Details

Other Experimental DetailsFlow cytometry analysis of Thrombomodulin in mouse PBMCs. 10^6 cells were incubated with 10 uL of Rat anti-Mouse PE-conjugated Thrombomodulin/BDCA-3 antibody (red) or Isotype Control IgG (black). Blocking: 1% BSA-PBS.

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