Mouse Thrombomodulin/BDCA-3 Antibody

(1 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Thrombomodulin/BDCA-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Thrombomodulin is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 461714
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Thrombomodulin/BDCA-3
    Leu17-Ser517
    Accession # P15306
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse Thrombomodulin/THBD/CD141 (Catalog # 3894-PA)
    under non-reducing conditions only
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Thrombomodulin/BDCA-3 in bEnd.3 Mouse Cell Line. Thrombomodulin/BDCA-3 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Mouse Thrombomodulin/BDCA-3 Monoclonal Antibody (Catalog # MAB3894) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
Thrombomodulin/BDCA-3 in Mouse Heart. Thrombomodulin/BDCA-3 was detected in perfusion fixed frozen sections of mouse heart using 25 µg/mL Mouse Thrombomodulin/BDCA-3 Monoclonal Antibody (Catalog # MAB3894) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Thrombomodulin/BDCA‑3 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Mouse Thrombomodulin/BDCA‑3 Monoclonal Antibody (Catalog # MAB3894, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thrombomodulin/BDCA-3

Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. The deduced amino acid sequence of mouse THBD predicts a signal peptide (aa 1 to 16) and a mature chain (aa 17 to 577) that consists of the following domains: C-type lectin (aa 31 to 167), EGF-like (aa 240 to 280, aa 283 to 323, aa 324 to 362, aa 364 to 404, aa 405 to 439, and aa 440 to 480), transmembrane (aa 518 to 541) and cytoplasmic (aa 542 to 577) (1). The R&D Systems rmTHBD consists of aa 17 to 517, corresponding to the extracellular portion of the type I membrane protein. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (2‑4). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal amino acid residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (5). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (6).

  • References:
    1. Dittman, W.A. and P.W. Majerus (1989) Nucleic Acids Res. 17:802.
    2. Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. 24:1374.
    3. Wu, K.K. et al. (2000) Ann Med. 32:73.
    4. Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
    5. Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
    6. Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.
  • Entrez Gene IDs:
    7056 (Human); 21824 (Mouse); 83580 (Rat)
  • Alternate Names:
    BDCA3; BDCA-3; CD141 antigen; CD141; Fetomodulin; THBD; THRM; Thrombomodulin; TM
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Heme triggers TLR4 signaling leading to endothelial cell activation and vaso-occlusion in murine sickle cell disease.
    Authors: Belcher J, Chen C, Nguyen J, Milbauer L, Abdulla F, Alayash A, Smith A, Nath K, Hebbel R, Vercellotti G
    Blood, 2014;123(3):377-90.
    Species: Mouse
    Sample Type: In Vivo
    Application: Bioassay
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Rat HRP-DAB Cell & Tissue Staining Kit

CTS017 5
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2B Isotype Control

Ctrl MAB0061R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Mouse Thrombomodulin/BDCA-3 Antibody MAB3894
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Flow Cytometry Mouse Thrombomodulin/BDCA-3 Antibody MAB3894
Flow Cytometry: Mouse Thrombomodulin/BDCA-3 Antibody [MAB3894]

Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)
SpeciesMouse

Other Experimental Details

Other Experimental Details106 Mouse PBMCs were incubated with 2.5 ug of Rat anti-Mouse Thrombomodulin/BDCA-3 antibody (pink) or Isotype Control IgG (black), followed by PE-conjugated Anti-Rat secondary antibody.

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