Mouse Thrombomodulin/BDCA-3 Antibody (461714)

R&D Systems | Catalog # MAB3894

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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Bioassay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 461714
View all Thrombomodulin/BDCA-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Thrombomodulin/BDCA-3
Leu17-Ser517
Accession # P15306

Specificity

Detects mouse Thrombomodulin/BDCA-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Thrombomodulin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Thrombomodulin/BDCA-3 Antibody (461714)

Thrombomodulin/BDCA-3 antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC).

Thrombomodulin/BDCA-3 in bEnd.3 Mouse Cell Line.

Thrombomodulin/BDCA-3 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Mouse Thrombomodulin/BDCA-3 Monoclonal Antibody (Catalog # MAB3894) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Thrombomodulin/BDCA-3 antibody in Mouse Heart by Immunohistochemistry (IHC-P) by Immunohistochemistry (IHC-Fr).

Thrombomodulin/BDCA-3 in Mouse Heart.

Thrombomodulin/BDCA-3 was detected in perfusion fixed frozen sections of mouse heart using 25 µg/mL Mouse Thrombomodulin/BDCA-3 Monoclonal Antibody (Catalog # MAB3894) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Thrombomodulin/BDCA‑3 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Thrombomodulin/BDCA‑3 in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Mouse Thrombomodulin/BDCA‑3 Monoclonal Antibody (Catalog # MAB3894, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).

Applications for Mouse Thrombomodulin/BDCA-3 Antibody (461714)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed bEND.3 mouse endothelioma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney and heart

Western Blot

1 µg/mL
Sample: Recombinant Mouse Thrombomodulin/THBD/CD141 (Catalog # 3894-PA)
under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 5 using MAB3894 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombomodulin/BDCA-3

Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. The deduced amino acid sequence of mouse THBD predicts a signal peptide (aa 1 to 16) and a mature chain (aa 17 to 577) that consists of the following domains: C-type lectin (aa 31 to 167), EGF-like (aa 240 to 280, aa 283 to 323, aa 324 to 362, aa 364 to 404, aa 405 to 439, and aa 440 to 480), transmembrane (aa 518 to 541) and cytoplasmic (aa 542 to 577) (1). The R&D Systems rmTHBD consists of aa 17 to 517, corresponding to the extracellular portion of the type I membrane protein. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (2‑4). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal amino acid residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (5). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (6).

References

  1. Dittman, W.A. and P.W. Majerus (1989) Nucleic Acids Res. 17:802.
  2. Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. 24:1374.
  3. Wu, K.K. et al. (2000) Ann Med. 32:73.
  4. Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
  5. Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
  6. Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.

Alternate Names

BDCA-3, BDCA3, CD141, Fetomodulin, THBD, THRM

Entrez Gene IDs

7056 (Human); 21824 (Mouse); 83580 (Rat)

Gene Symbol

THBD

UniProt

P15306

Additional Thrombomodulin/BDCA-3 Products

Product Documents for Mouse Thrombomodulin/BDCA-3 Antibody (461714)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Thrombomodulin/BDCA-3 Antibody (461714)

For research use only

Citations for Mouse Thrombomodulin/BDCA-3 Antibody (461714)

Customer Reviews for Mouse Thrombomodulin/BDCA-3 Antibody (461714) (2)

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  • Mouse Thrombomodulin/BDCA-3 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Astrocytes
    Species: Mouse
    Verified Customer | Posted 02/10/2022
    Mouse Thrombomodulin/BDCA-3 Antibody (461714) MAB3894
  • Mouse Thrombomodulin/BDCA-3 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Mouse
    Verified Customer | Posted 03/22/2017
    106 Mouse PBMCs were incubated with 2.5 ug of Rat anti-Mouse Thrombomodulin/BDCA-3 antibody (pink) or Isotype Control IgG (black), followed by PE-conjugated Anti-Rat secondary antibody.
    Mouse Thrombomodulin/BDCA-3 Antibody (461714) MAB3894

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Protocols

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