Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit

R&D Systems | Catalog # MTHBD0

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)

Sensitivity

19.1 pg/mL

Assay Range

46.9-3000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit

The Quantikine Mouse Thrombomodulin/BDCA-3 immunoassay is a 4.5 hour solid-phase ELISA designed to measure Thrombomodulin in mouse cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse Thrombomodulin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Thrombomodulin. Results obtained using natural mouse Thrombomodulin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Thrombomodulin.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Natural and recombinant mouse Thrombomodulin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 108 388 1159 121 380 1155
Standard Deviation 6.67 16.0 44.8 16.0 38.1 136
CV% 6.2 4.1 3.9 13.2 10.0 11.8

Recovery for Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit

The recovery of mouse Thrombomodulin spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 91-115

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse Thrombomodulin in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. All samples were diluted prior to assay.

Mouse Thrombomodulin/BDCA-3 ELISA Linearity

Scientific Data Images for Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit

Mouse Thrombomodulin/BDCA-3 ELISA Standard Curve

Mouse Thrombomodulin/BDCA-3 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Thrombomodulin/BDCA-3

Thrombomodulin (TM), also known as CD141 and BDCA3, is a transmembrane expressed on vascular endothelial cells (EC), arterial smooth muscle cells, monocytes, and macrophages. It binds Thrombin and enhances the Thrombin-mediated activation of the anti-coagulant Protein C and the anti-fibrinolytic TAFI/Carboxypeptidase B2. Thrombomodulin also inhibits the ability of Thrombin to activate several pro-coagulant proteins (e.g. Fibrinogen, Factor V, Factor XIII, PAR-1). Thrombomodulin exerts multiple anti-inflammatory effects and reduces the severity of disease involving chronic inflammation. In cancer, Thrombomodulin suppresses tumor angiogenesis, growth, and epithelial-mesenchymal transition. Soluble fragments of Thrombomodulin are elevated in the serum, urine, and synovial fluid during coagulation disorders, inflammation, and organ failure.

Alternate Names

BDCA-3, BDCA3, CD141, Fetomodulin, THBD, THRM

Entrez Gene IDs

7056 (Human); 21824 (Mouse); 83580 (Rat)

Gene Symbol

THBD

Additional Thrombomodulin/BDCA-3 Products

Product Documents for Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit

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Protocols

View specific protocols for Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit (MTHBD0):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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