TIM-1 (T cell immunoglobulin and mucin domain 1), also known as KIM-1 and HAVcr1, is expressed on many immune cell types and epithelial cells. It binds to phosphatidylserine (PS), LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with PS enables TIM-1 to mediate the phagocytosis and clearance of apoptotic cells. TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. TIM-1 serves as a cellular entry receptor for hepatitis A virus, Ebolavirus and Marburgvirus. Polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans. A soluble form of TIM-1 is elevated in the urine during nephropathy.
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Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
R&D Systems | Catalog # MKM100
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Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (10 µL)
Sensitivity
2.18 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
The Quantikine Mouse TIM-1 Immunoassay is a 3.5 hour solid phase ELISA designed to measure mouse TIM-1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse TIM-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse TIM-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TIM-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse TIM-1. The antibody components of this kit were raised against the short splice form of mouse TIM-1 (isoform 2) which has a 23 amino acid deletion following Pro182. This immunoassay detects both the short and long forms of mouse TIM-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Urine - Samples were evaluated for the presence of mouse TIM-1 in this assay
ND=Non-Detectable
| NSA Strain | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Serum (n=20) | 17.2 | 90 | ND-42.5 |
| EDTA plasma (n=20) | 25.6 | 100 | 7.87-174 |
| Heparin plasma (n=20) | 18.0 | 95 | ND-42.8 |
| Urine (n=20) | 2248 | 100 | 456-8048 |
Serum samples from two Balb/c strain mice were evaluated in this assay and measured
27.5 pg/mL and 26.9 pg/mL. Additionally, serum samples from two C57 strain mice were
evaluated in this assay and measured 20.4 pg/mL and 14.4 pg/mL.
Urine samples from two Balb/c strain mice and one C57 strain mouse were evaluated in this
assay and measured 143,000 pg/mL, 159,000 pg/mL, and 726 pg/mL respectively.
Cell Culture Supernates - Kidneys from mice were removed, rinsed in 1X PBS, and kept on
ice in 1X PBS. Kidneys were then homogenized using a tissue homogenizer and seeded into
media containing RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine,
100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured for the times
indicated. Aliquots of the cell culture supernates were removed and assayed for levels of
mouse TIM-1.
| Tissue Type | (pg/mL) |
| NSA mouse kidney (1 day) | 1006 |
| NSA mouse kidney (3 days) | 756 |
| Balb/c mouse kidney (2 days) | 1679 |
| C57 mouse kidney (2 days) | 24 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 24.2 | 61.8 | 207 | 23.2 | 60.0 | 190 |
| Standard Deviation | 0.8 | 1.6 | 6.8 | 2.5 | 4.2 | 13.2 |
| CV% | 3.3 | 2.6 | 3.3 | 10.8 | 7.0 | 6.9 |
Recovery for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
The recovery of TIM-1 spiked into various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 91-106 |
| EDTA Plasma (n=4) | 89 | 88-91 |
| Heparin Plasma (n=4) | 87 | 86-89 |
| Serum (n=4) | 95 | 93-97 |
| Urine (n=4) | 102 | 94-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse TIM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
Mouse KIM-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TIM-1/KIM-1/HAVCR
Long Name
T Cell Immunoglobulin Mucin-1
Alternate Names
CD365, HAVCR1, KIM-1, TIM1
Gene Symbol
HAVCR1
Additional TIM-1/KIM-1/HAVCR Products
Product Documents for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
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Protocols
View specific protocols for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit (MKM100):
Refer to the product for complete assay procedure.
The conjugate must remain at 2-8 °C during use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour on the benchtop.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL cold Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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