Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 339319
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIM‑5
Phe22-Gly161
Accession # NP_001156829

Specificity

Detects mouse TIM-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse TIM-1, -2, -3, -4, or -6 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse TIM‑5 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TIM‑5 under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIM-5

Mouse TIM-5 (T cell immunoglobulin mucin-5; also hepatitis A virus cellular receptor 1-like) is presumably a member of the TIM family of immune regulatory proteins. Based on other TIM molecules, it will be a type I transmembrane protein with one V-type Ig-like domain and a Ser/Thr-rich mucin region. Mouse TIM-5 has 47% aa identity to mouse TIM-2.

Long Name

T Cell Immunoglobulin Mucin-5

Alternate Names

TIM5

Entrez Gene IDs

210535 (Mouse)

Gene Symbol

GM12169

UniProt

Additional TIM-5 Products

Product Documents for Mouse TIM‑5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TIM‑5 Antibody

For research use only

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FAQs

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