Mouse TIMP-4 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB7667-SP
MAB7667-100
Product Details
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Mouse TIMP-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TIMP-4 in direct ELISAs.
Source
Recombinant Monoclonal Rat IgG1 Clone # 866106R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIMP-4
Met1-Pro224
Accession # Q9JHB3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human/Mouse TIMP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF974).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse TIMP-4 DuoSet ELISA Kit (Catalog # DY7667-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-4

Tissue Inhibitors of Metalloproteinases (TIMPs) are a family of secreted proteins that regulate the activation and proteolytic activity of the zinc enzymes known as Matrix Metalloproteinases (MMPs). There are four known members of the family, TIMP-1, -2, -3, and -4. TIMP-4 is produced by a wide range of tissues, particularly brain, heart, ovary and skeletal muscle (1, 2). Limited studies have shown that TIMP-4 has a tumor suppressive effect against Wilm’s tumor, exhibits negative correlation with glioma maligancy and is found in breast carcinoma cells (3, 5). TIMP-4 inhibits MMP-mediated proteolysis by forming a noncovalent binary complex with the MMP active site through its N-terminal domain. In addition, it binds to the hemopexin-like domain of proMMP-2 through its C-terminal domain in a manner similar to TIMP-2 (6). However, unlike TIMP-2, TIMP-4 does not promote proMMP-2 activation by MT1-MMP (MMP-14) (7). Although TIMP-4 is a potent inhibitor of most MMPs, it is not an effective inhibitor of ADAMs, such as TACE (8, 9).

References
  1. Greene et al. (1996) J. Biol. Chem. 271:30375.
  2. Leco et al. (1997) FEBS Lett. 401:213.
  3. Geliker et al. (2001) Oncogene 20:4337.
  4. Groft et al. (2001) Br. J. Cancer 85:55.
  5. Hurst et al. (2001) Biochem. Biophys. Res. Comm. 281:166.
  6. Bigg et al. (1997) J. Biol. Chem. 272:15496.
  7. Hernandez-Barrantes et al. (2001) Biochem. Biophys. Res. Comm. 281:126.
  8. Amour et al. (1998) FEBS Lett. 435:39.
  9. Liu et al. (1997) J. Biol. Chem. 272:20479.
Long Name
Tissue Inhibitors of Metalloproteinases 4
Entrez Gene IDs
7079 (Human); 110595 (Mouse)
Alternate Names
metalloproteinase inhibitor 4; TIMP metallopeptidase inhibitor 4; TIMP4; TIMP-4; tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4

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