Key Product Details

Species Reactivity

Mouse

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG1 Clone # 866106R
View all TIMP-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIMP-4
Met1-Pro224
Accession # Q9JHB3

Specificity

Detects mouse TIMP-4 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse TIMP‑4 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human/Mouse TIMP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF974).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse TIMP-4 DuoSet ELISA Kit (Catalog # DY7667-05) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIMP-4

Tissue Inhibitors of Metalloproteinases (TIMPs) are a family of secreted proteins that regulate the activation and proteolytic activity of the zinc enzymes known as Matrix Metalloproteinases (MMPs). There are four known members of the family, TIMP-1, -2, -3, and -4. TIMP-4 is produced by a wide range of tissues, particularly brain, heart, ovary and skeletal muscle (1, 2). Limited studies have shown that TIMP-4 has a tumor suppressive effect against Wilm’s tumor, exhibits negative correlation with glioma maligancy and is found in breast carcinoma cells (3, 5). TIMP-4 inhibits MMP-mediated proteolysis by forming a noncovalent binary complex with the MMP active site through its N-terminal domain. In addition, it binds to the hemopexin-like domain of proMMP-2 through its C-terminal domain in a manner similar to TIMP-2 (6). However, unlike TIMP-2, TIMP-4 does not promote proMMP-2 activation by MT1-MMP (MMP-14) (7). Although TIMP-4 is a potent inhibitor of most MMPs, it is not an effective inhibitor of ADAMs, such as TACE (8, 9).

References

  1. Greene et al. (1996) J. Biol. Chem. 271:30375.
  2. Leco et al. (1997) FEBS Lett. 401:213.
  3. Geliker et al. (2001) Oncogene 20:4337.
  4. Groft et al. (2001) Br. J. Cancer 85:55.
  5. Hurst et al. (2001) Biochem. Biophys. Res. Comm. 281:166.
  6. Bigg et al. (1997) J. Biol. Chem. 272:15496.
  7. Hernandez-Barrantes et al. (2001) Biochem. Biophys. Res. Comm. 281:126.
  8. Amour et al. (1998) FEBS Lett. 435:39.
  9. Liu et al. (1997) J. Biol. Chem. 272:20479.

Long Name

Tissue Inhibitors of Metalloproteinases 4

Alternate Names

TIMP4

Entrez Gene IDs

7079 (Human); 110595 (Mouse)

Gene Symbol

TIMP4

UniProt

Q9JHB3

Additional TIMP-4 Products

Product Documents for Mouse TIMP‑4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TIMP‑4 Antibody

For research use only

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