Mouse TLR2 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1530F
Detection of TLR2 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (1)
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Mouse TLR2 Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TLR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse (rm) TLR1, recombinant human (rh) TLR2, rhTLR3, rhTLR4, or rmTLR6.
Source
Monoclonal Rat IgG2B Clone # 203325
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TLR2
Gln25-Leu590
Accession # Q9QUN7
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TLR2 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of TLR2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR2 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1530F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TLR2

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens (1-4). To date, there are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1, 3). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular nucleic acids) (3). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. Mouse Toll-like receptor-2 (TLR2) is a 97 kDa, 760 amino acid (aa) glycoprotein that contains a 563 aa extracellular region, a 21 aa TM segment, and a 176 aa cytoplasmic domain (5, 6). The extracellular region contains 16 leucine-rich repeats, while the cytoplasmic tail shows one 146 aa TIR domain. The receptor is expressed on a number of cell types including T cells ( alpha beta and gamma δ), monocytes, dendritic cells, neutrophils, B cells, endothelial cells, mast cells, NK cells, macrophages, and hepatocytes (1, 4, 5, 7, 8). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6 (1, 3, 4). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers recognize triacylated lipopeptides from a variety of microorganisms. The TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (9). Biglycan is also known to activate TLR2, but the context is unclear (8). Notably, in human, TLR2 also dimerizes with TLR10. But the TLR10 gene in mouse (but not rat) is mutationally inactive, and thus this complex is nonfunctional (10). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 11). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1, 3). The extracellular region of mouse TLR2 is 89%, 67%, 81%, and 65% aa identical to the equivalent region in rat, human, hamster, and canine, respectively.

 

References
  1. Wetzler, L. (2003) Vaccine 21:S2/55.
  2. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
  3. Dunne, A. and L. O’Neill (2005) FEBS. Lett. 579:3330.
  4. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  5. Matsuguchi, T. et al. (2000) Blood 95:1378.
  6. Meng, G. et al. (2005) Immunol. Lett. 98:200.
  7. Flo, T. et al. (2001) J. Leukoc. Biol. 69:474.
  8. Schaefer, L. et al. (2005) J. Clin. Invest. 115:2223.
  9. Akira, S. (2003) Curr. Opin. Immunol. 15:5.
  10. Hasan, U. et al. (2005) J. Immunol. 174:2942.
  11. Yamamoto M. et al. (2002) Nature 420:324.
Long Name
Toll-like Receptor 2
Entrez Gene IDs
7097 (Human); 24088 (Mouse); 310553 (Rat)
Alternate Names
CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2

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Citation for Mouse TLR2 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Morphine Inhibits Murine Dendritic Cell IL-23 Production by Modulating Toll-like Receptor 2 and Nod2 Signaling.
    Authors: Wang J, Ma J, Charboneau R
    J. Biol. Chem., 2011;286(12):10225-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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