Mouse TRAM/TICAM2 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB43481N Datasheet / COA / SDS

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FAB43481N-100UG

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All TRAM/TICAM2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TRAM/TICAM2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TRAM/TICAM2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRAM or rhTRIF is observed.

Source

Monoclonal Rat IgG2b Clone # 757712

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse TRAM/TICAM2
Met1-Ala232
Accession # Q8BJQ4

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TRAM/TICAM2

The innate and adaptive immune responses depend on systems that link cell surface surveillance receptor signals to cytoplasmic proteins such as kinases, adaptors, and transcription factors. Toll-like receptors (TLR) recognize different pathogen associated molecular patterns (PAMPs), and initiate a signaling cascades mediated by a Toll/interleukin-1 receptor (TIR) domain-containing adaptor proteins such as MyD88, TIRAP/MAL, and TRIF. Mouse TRIF-related adaptor molecule (TRAM), is a 232 amino acid, 26 kDa (predicted), ubiquitously expressed member of the TIR domain-containing adaptor family. TRAM, also known as TIR domain-containing adapter protein 2 (TICAM2) and TIR domain-containing protein (TIRP), contains a central Toll/interleukin-1 receptor (TIR) domain that is most similar to that of TRIF. TRAM plays an essential role in the MyD88-independent signaling of TLR4 by binding members of the IRAK family, ultimately leading to the activation of NF kappa B. Mouse TRAM shares 75% and 77% amino acid sequence identity with human and rat TRAM, respectively.

Long Name

TRIF-related Adapter Molecule

Entrez Gene IDs

353376 (Human); 225471 (Mouse); 364867 (Rat)

Alternate Names

MGC129876; MGC129877; Putative NF-kappa-B-activating protein 502; TICAM2; TICAM-2TIR domain-containing adapter molecule 2; TIRAP3; TIRP; TIRPcytoplasmic adaptor; toll/interleukin-1 receptor (TIR) domain-containing adapter protein; Toll/interleukin-1 receptor domain-containing protein; toll-like receptor adaptor molecule 2; Toll-like receptor adaptor protein 3; TRAM; TRAMNF-kappa-B-activating protein 502; Trif; TRIF-related adapter molecule; TRIF-related adaptor molecule