Mouse TRAM/TICAM2 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TRAM/TICAM2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRAM or rhTRIF is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 757712
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse TRAM/TICAM2
    Met1-Ala232
    Accession # Q8BJQ4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
TRAM/TICAM2 in C2C12 Mouse Cell Line. TRAM/TICAM2 was detected in immersion fixed C2C12 mouse myoblast cell line using Rat Anti-Mouse TRAM/TICAM2 Monoclonal Antibody (Catalog # MAB43481) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAM/TICAM2

The innate and adaptive immune responses depend on systems that link cell surface surveillance receptor signals to cytoplasmic proteins such as kinases, adaptors, and transcription factors. Toll-like receptors (TLR) recognize different pathogen associated molecular patterns (PAMPs), and initiate a signaling cascades mediated by a Toll/interleukin-1 receptor (TIR) domain-containing adaptor proteins such as MyD88, TIRAP/MAL, and TRIF. Mouse TRIF-related adaptor molecule (TRAM), is a 232 amino acid, 26 kDa (predicted), ubiquitously expressed member of the TIR domain-containing adaptor family. TRAM, also known as TIR domain-containing adapter protein 2 (TICAM2) and TIR domain-containing protein (TIRP), contains a central Toll/interleukin-1 receptor (TIR) domain that is most similar to that of TRIF. TRAM plays an essential role in the MyD88-independent signaling of TLR4 by binding members of the IRAK family, ultimately leading to the activation of NF kappa B. Mouse TRAM shares 75% and 77% amino acid sequence identity with human and rat TRAM, respectively.

  • Long Name:
    TRIF-related Adapter Molecule
  • Entrez Gene IDs:
    353376 (Human); 225471 (Mouse); 364867 (Rat)
  • Alternate Names:
    MGC129876; MGC129877; Putative NF-kappa-B-activating protein 502; TICAM2; TICAM-2TIR domain-containing adapter molecule 2; TIRAP3; TIRP; TIRPcytoplasmic adaptor; toll/interleukin-1 receptor (TIR) domain-containing adapter protein; Toll/interleukin-1 receptor domain-containing protein; toll-like receptor adaptor molecule 2; Toll-like receptor adaptor protein 3; TRAM; TRAMNF-kappa-B-activating protein 502; Trif; TRIF-related adapter molecule; TRIF-related adaptor molecule
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