Detects mouse TREML2/TLT-2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
mouse (rm) TREM-1, -2, -2b, -3, -4, rmTREML1/TLT-1, rmTREML4/TLT-4, or
recombinant human (rh) NKp44 is observed.
Monoclonal Rat IgG2A Clone # 656906
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant mouse TREML2/TLT-2 His25-Ala270 Accession # Q2LA85
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TREML2 in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Rat Anti-Mouse TREML2/TLT‑2 Monoclonal Antibody (Catalog # MAB6707, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TREML2, also called TLT-2 (Trem-like transcript 2) is a 33 kDa (predicted) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins. Mouse TLT-2 is synthesized as a 329 amino acid (aa) precursor that contains a 24 aa signal sequence, a 246 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 38 aa cytoplasmic tail. Within the ECD are an Ig-like V-type domain (aa 25-126) and one potential site for N-linked glycosylation. Mature mouse TLT-2 shares 49% aa sequence identity with mature human TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines. TLT-2 is also expressed constitutively on CD8+ T cells.
Triggering Receptor Expressed on Myeloid Cells-like 2
Entrez Gene IDs:
79865 (Human); 328833 (Mouse)
C6orf76; dJ238O23.1; FLJ13693; MGC149715; MGC149716; TLT2; TLT-2; TREML2; trem-like transcript 2 protein; triggering receptor expressed on myeloid cells-like 2; triggering receptor expressed on myeloid cells-like protein 2; UNQ6268/PRO20473
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