Mouse TROY/TNFRSF19 Antibody

Catalog # Availability Size / Price Qty
MAB723
MAB723-SP
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Mouse TROY/TNFRSF19 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TROY/TNFRSF19 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) 4‑1BB, rmBAFF R, recombinant human RELT, rmCD3, rhDR6, rmDR3, rhTRADD, rmEDAR, recombinant rat FAS, rmFAS, rmGITR, rmHVEM, rmLymphotoxin beta R, rmNGF R, rmOPG, rmRANK, rmTNF RII, rmDcTRAIL R2, rmDcTRAIL RI, rmOX40, rhTRAIL R3, rhTNF RII, rmTWEAK, rmTRAIL, rmCD27, rmCD40, rhTNF RI, or rmTNF RI is observed.
Source
Monoclonal Rat IgG2A Clone # 323606
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TROY/TNFRSF19
Glu30-Leu170
Accession # Q9JLL3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse TROY/TNFRSF19 Fc Chimera (Catalog # 723-TR) under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TROY/TNFRSF19

TROY (also known as TAJ and TNFRSF19) is a 45‑55 kDa member of the TNF receptor superfamily of molecules (1‑4). It is widely expressed in the embryo, but shows limited expression in adult. In particular, embryonic TROY is found in embryonic epithelium, neural tube, and mesenchyme, and developing neurons of the hippocampus and thalamus (5, 6). In the adult, TROY is found in the nervous system associated with dorsal root and retinal ganglia neurons, astrocytes, and microglia, and in hair follicles where it is expressed by dermal papilla matrix cells (4, 7, 8, 9). In human, TROY has also been found on melanoma cells, not normal melanocytes (10). TROY is reportedly a component of the Nogo-A receptor, taking the place of p75 in a membrane-bound LINGO-1:NgR1:TROY complex (6  8, 9). This complex purportedly mediates axon repulsion initiated by one of five myelin-associated inhibitory factors (OMpg; CD100; Ephrin B3; Nogo-A; MAG) (6, 11). In the hair follicle, TROY binds LTa and acts with Eda to drive secondary hair follicle development (4, 12). Mature human TROY is a 394 amino acid (aa) type I transmembrane protein (1‑3). It possesses a 141 aa extracellular region (aa 30‑170) that contains three 40 aa TNFR cysteine motifs, and an extended 232 aa cytoplasmic domain (aa 192‑423). There is one potential isoform that shows a two aa substitution for aa 416 ‑ 423. The extracellular domains of human and mouse TROY share 90% aa identity.

References
  1. Hu, S. et al. (1999) Genomics 62:103.
  2. Eby, M.T. et al. (2000) J. Biol. Chem. 275:15336.
  3. Kojima, T. et al. (2000) J. Biol. Chem. 275:20742.
  4. Hashimoto, T. et al. (2008) Cell Cycle 7:106.
  5. Pispa, J. et al. (2003) Gene Exp. Patterns 3:675.
  6. Mi, S. (2008) Cytokine Growth Factor Rev. 19:245.
  7. Satoh, J. et al. (2007) Neuropathol. Appl. Neurobiol. 33:99.
  8. Shao, Z. et al. (2005) Neuron 45:353.
  9. Park, J.B. et al. (2000) Neuron 45:345.
  10. Spanjaard, R.A. et al. (2007) Int. J. Cancer 120:1304.
  11. Venkatesh, K. et al. (2007) J. Cell Biol. 177:393.
  12. Pispa, J. et al. (2008) Hum. Mol. Genet. 17:3380.
Long Name
Tumor Necrosis Factor Receptor Superfamily, Member 19
Entrez Gene IDs
55504 (Human); 29820 (Mouse)
Alternate Names
TAJ; TAJ-alpha; TAJToxicity and JNK inducer; TNFRSF19; TRADE; TROY; TROYtumor necrosis factor receptor superfamily member 19; tumor necrosis factor receptor superfamily, member 19

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