Mouse TROY/TNFRSF19 Antibody

Catalog # Availability Size / Price Qty
AF723
AF723-SP
Product Details
Citations (1)
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Mouse TROY/TNFRSF19 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TROY/TNFRSF19 in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human TAJ is observed and less than 0.2% cross-reactivity with recombinant mouse (rm) EDAR, rmCD27, rmFas, rmRANK, rmTNF RI, and rmTNF RII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TROY/TNFRSF19
Glu30-Leu170
Accession # Q9JLL3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TROY/TNFRSF19 Fc Chimera (Catalog # 723-TR)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse brain (cortex)

Mouse TROY/TNFRSF19 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse TROY/TNFRSF19 Biotinylated Antibody (Catalog # BAF723)

Standard: Recombinant Mouse TROY/TNFRSF19 Fc Chimera Protein, CF (Catalog # 723-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TROY/TNFRSF19

TROY, also named Toxicity and JNK inducer (TAJ) and TNFRSF19, is a novel member of the tumor necrosis factor receptor superfamily. The protein putatively encoded by the TROY mRNA is a 416 amino acid (aa) residue type I membrane protein with a 29 aa signal peptide, a 141 aa extracellular domain, a 23 aa transmembrane domain and a 223 aa cytoplasmic domain. Splice variants of mouse TROY (lacking the transmembrane and cytoplasmic domains or with only a 21 aa cytoplasmic tail), have also been identified. Like other members of the TNFRSF, TROY contains characteristic cysteine-rich motifs in the extracellular domain. The cytoplasmic domain of TROY does not have the death domain present in some TNFRSF members, but does contain a major TRAF2 (tumor necrosis factor
receptor‑associated factor 2)-binding consensus sequence. Mouse TROY shares 92% aa sequence identity with human TROY in their extracellular domains. The two proteins also have 57% homology in their cytoplasmic tails. Among TNFRSF members, TROY is most related to Edar, sharing 33% identity in the extracellular domain. Over‑expression of TROY has been reported to activate nuclear factor NF kappa B and c-Jun N-terminal kinase (JNK) pathways. TROY mRNA is exclusively expressed in the epithelia of various tissues in mouse day 13.5 embryos. In neonatal mice, expression of TROY is predominantly in hair follicles and in neuron-like cells in the cerebrum.

References
  1. Kojima, T. et al. (2000) J. Biol. Chem. 275:20742.
  2. Eby, M.T. et al. (2000) J. Biol. Chem. 275:15336.
  3. Hu, S. et al. (1999) Genomics 62:103.
Long Name
Tumor Necrosis Factor Receptor Superfamily, Member 19
Entrez Gene IDs
55504 (Human); 29820 (Mouse)
Alternate Names
TAJ; TAJ-alpha; TAJToxicity and JNK inducer; TNFRSF19; TRADE; TROY; TROYtumor necrosis factor receptor superfamily member 19; tumor necrosis factor receptor superfamily, member 19

Product Datasheets

Citation for Mouse TROY/TNFRSF19 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Myelin-associated proteins block the migration of olfactory ensheathing cells: an in vitro study using single-cell tracking and traction force microscopy.
    Authors: Nocentini S, Reginensi D, Garcia S, Carulla P, Moreno-Flores MT, Wandosell F, Trepat X, Bribian A, del Rio JA
    Cell. Mol. Life Sci., 2011;69(10):1689-703.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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