Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Tryptase beta-1/MCP-7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Tryptase beta ‑1/MCP‑7/Mcpt7
Ile29-Phe273
Accession # Q02844

Specificity

Detects mouse Tryptase beta ‑1/MCP‑7/Mcpt7 in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant mouse MCPT1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Tryptase beta-1/MCP-7/Mcpt7 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Tryptase  beta ‑1/MCP‑7/Mcpt7 (Catalog # 1937-SE)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tryptase beta-1/MCP-7

Tryptase beta -1 is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7 (1). It is stored in the secretory granules of mouse mast cells (2). It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma (3). The deduced amino acid sequence for mouse Tryptase beta -1 consists of a signal peptide (residues 1‑18), a pro region (residues 19‑28) and the mature chain (residue 29‑273). The mature chain was expressed and purified.

References

  1. McNeil, H.P. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11174.
  2. Matsumoto, R et al. (1995) J. Biol. Chem. 270:19524.
  3. Huang, C. et al. (1997) J. Biol. Chem. 272:31885.

Long Name

Mast Cell Protease 7

Alternate Names

Mast Cell Tryptase, MCP-7, Mcp7, Mcpt7, TPSB1, TRYB1, Tryptase beta1

Entrez Gene IDs

100503895 (Mouse)

Gene Symbol

TPSAB1

UniProt

Q02844

Additional Tryptase beta-1/MCP-7 Products

Product Documents for Mouse Tryptase beta-1/MCP-7/Mcpt7 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Tryptase beta-1/MCP-7/Mcpt7 Biotinylated Antibody

For research use only

Citations for Mouse Tryptase beta-1/MCP-7/Mcpt7 Biotinylated Antibody

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