Mouse Tryptase  beta -1/MCP-7/Mcpt7 Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Tryptase beta ‑1/MCP‑7/Mcpt7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) TPSB2 is observed, 5% cross-reactivity with rhTPS1 is observed, and less than 1% cross-reactivity with rhTPSG1 and recombinant mouse MCPT1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Tryptase beta ‑1/MCP‑7/Mcpt7
    Ile29-Phe273
    Accession # Q02844
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tryptase beta-1/MCP-7/Mcpt7

Tryptase beta -1 is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7 (1). It is stored in the secretory granules of mouse mast cells (2). It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma (3). The deduced amino acid sequence for mouse Tryptase beta -1 consists of a signal peptide (residues 1‑18), a pro region (residues 19‑28) and the mature chain (residue 29‑273). The mature chain was expressed and purified.

  • References:
    1. McNeil, H.P. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11174.
    2. Matsumoto, R et al. (1995) J. Biol. Chem. 270:19524.
    3. Huang, C. et al. (1997) J. Biol. Chem. 272:31885.
  • Entrez Gene IDs:
    17230 (Mouse)
  • Alternate Names:
    MCP-7; Mcpt7; TPSB1; Tryptase beta1; Tryptase beta-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Epidermal RAF prevents allergic skin disease
    Elife, 2016;5(0):.
  2. Blockade of mast cell activation reduces cutaneous scar formation.
    Authors: Chen L, Schrementi M, Ranzer M, Wilgus T, DiPietro L
    PLoS ONE, 2014;9(1):e85226.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Phagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation.
    Authors: von Kockritz-Blickwede M, Goldmann O, Thulin P, Heinemann K, Norrby-Teglund A, Rohde M, Medina E
    Blood, 2008;111(6):3070-80.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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