Mouse Tryptase beta-1/MCP-7 Antibody

Detection of Tryptase beta-1/Mcpt7 by Immunohistochemistry

ROS-resistant CaMKII protects against allergen-induced mast cell activation.(A–C) Analyses of beta -hexosaminidase (A, n = 6), histamine release (B, n = 4), and cytokines and chemokines (C, n = 6) in supernatants of DNP-activated BMMCs derived from WT and CaMKII MM-VV delta mice. (D) Representative immunofluorescence images of dorsal skin sections and fluorescence analysis of ox-CaMKII staining in the skin mast cells of patients with AD and controls. Scale bar: 100 μm. (E) Quantitative analyses for total number of ox-CaMKII+ mast cells (n = 10). Data represent mean ± SEM of 2 independent experiments. Data were compared using a 2-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://insight.jci.org/articles/view/152559), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Tryptase beta-1/Mcpt7 by Immunohistochemistry

DCIR mediates cockroach allergen binding and uptake.(A and B) Representative immunofluorescence images of dorsal skin sections and fluorescence analysis of DCIR staining in the skin tissues of patients with AD and controls (n = 8). Scale bar: 100 μm. (C) Quantification analysis of DCIR+tryptase+ cells in the lesion skin of patients with AD and controls. (D) Flow cytometry analysis of DCIR expression in human mast cell line cKit+Fc epsilon RI+ LAD2 cells. (E) Scheme of experimental protocol for the direct bindings of human recombinant DCIR (hrDCIR) to BSA, CRE, and Man-BSA. (F) Direct binding of different doses of hrDCIR (0–5.0 μg/mL) to BSA, CRE, or Man-BSA (n = 3). (G) Representative immunofluorescence images of FITC-CRE uptake by LAD2. Scale bar: 15 μm. (H and I) Flow cytometry analysis (H) and quantification (I) of FITC-CRE uptake at different doses (1–500 ng/mL) by LAD2 cells (n = 3). (J) Inhibition of FITC-CRE uptake in LAD2 cells pretreated with DCIR neutralizing antibody ( alpha -DCIR) or IgG isotype (n = 4). Data represent mean ± SEM of 2 independent experiments. Data in B, C, and I were compared using a 2-tailed Student’s t test. Data in F and J were compared by 2-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://insight.jci.org/articles/view/152559), licensed under a CC-BY license. Not internally tested by R&D Systems.