Mouse TSLP R APC-conjugated Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TSLP R in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TSLP R
    Ala20-Leu233
    Accession # BAA92159
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TSLP R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse TSLP R APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5461A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TSLP R

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The TSLP R cDNA encodes a 359 amino acid (aa) residue type 1 membrane protein with a 24 aa residue signal peptide, a 206 aa residue extracellular domain that showed 24% sequence identity with the mouse common gamma receptor, a 23 aa residue transmembrane domain, and a 106 aa residue cytoplasmic domain. The cytoplasmic domain of TSLP R contains a membrane-proximal box1 motif which is known to be important for association with JAKs. An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3, 4).

  • References:
    1. Fujio, K. et al. (2000) Blood 95:2204.
    2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
    3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
    4. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
  • Long Name:
    Thymic Stromal Lymphopoietin Receptor
  • Entrez Gene IDs:
    64109 (Human); 57914 (Mouse); 171499 (Rat)
  • Alternate Names:
    CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP R; TSLP receptor; TSLPR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Deguelin Attenuates Allergic Airway Inflammation via Inhibition of NF-?b Pathway in Mice
    Authors: Z Bao, P Zhang, Y Yao, G Lu, Z Tong, B Yan, L Tu, G Yang, J Zhou
    Int. J. Biol. Sci., 2017;13(4):492-504.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG APC-conjugated Antibody

Ctrl IC108A 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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