Mouse USP2 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF5804V Datasheet / COA / SDS

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AF5804V-100UG

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Mouse USP2 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse USP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human USP2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse USP2 isoform 2
Lys47-Met396
Accession # NP_932759

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: USP2

USP2 (Ubiquitin-specific-processing protease 2; also Ubiquitin carboxy-terminal hydrolase 2) is a cytosolic member of the peptidase C19 family of enzymes. It is widely expressed and serves to deubiquitinate select target substrates. USP2 effects are determined by its targets. In cells where it increases Mdm2, p53 is degraded and apoptosis is blocked. In cells where it increases Itch concentration, Flip activity is interrupted and apoptosis is promoted. USP2 is reported to form homo‑oligomers. There are at least two isoform variants. Each is approximately 70 kDa in size, with one showing a 266 aa, and another a 272 aa substitution for aa 1‑49. Over aa 47‑396, mouse USP2 shares 96% and 99% aa identity with human and rat USP2, respectively.

Long Name

Ubiquitin-specific Protease 2

Entrez Gene IDs

9099 (Human); 53376 (Mouse); 115771 (Rat)

Alternate Names

Deubiquitinating enzyme 2; EC 3.1.2.15; EC 3.4.19.12,41 kDa ubiquitin-specific protease; ubiquitin specific peptidase 2; ubiquitin specific protease 12; ubiquitin specific protease 2; ubiquitin specific protease 9; ubiquitin thioesterase 2; Ubiquitin thiolesterase 2; Ubiquitin-specific-processing protease 2; UBP41; UBP41ubiquitin carboxyl-terminal hydrolase 2; USP2; USP9