Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Immunogen

E. coli-derived recombinant mouse UTF1
Lys50-Ser171
Accession # Q6J1H4

Specificity

Detects mouse UTF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human UTF1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse UTF1 Alexa Fluor® 350‑conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

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Spectra Viewer

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: UTF1

UTF1 (Undifferentiated embryonic cell Transcription Factor 1) is a 47-49 kDa member of Leu-zipper family of proteins. It is expressed in the early embryonic inner cell mass and primitive ectoderm, and disappears following the formation of the primitive streak. In this regard, UTF1 has been shown to be chromatin-associated and to participate in the proper differentiation of embryonic stem cells. It also is expressed in basal keratinocytes, and hair follicle matrix cells. UTF1 acts as a transcriptional coactivator of ATF2 (Activating Transcription factor 2), a bZIP family protein that interacts with CRE in gene promoters. Mouse UTF1 is 399 amino acids (aa) in length. It contains two conserved domains (aa 52-167 and aa 269-332), the latter which is key to ATS2 activation. There is one alternative start site at Met43 that generates a 39 kDa isoform. Over aa 18-423, mouse UTF1 shares 81% and 95% aa sequence identity with human and rat UTF1, respectively.

Long Name

Undifferentiated Embryonic Cell Transcription Factor 1

Alternate Names

undifferentiated embryonic cell transcription factor 1

Entrez Gene IDs

8433 (Human); 22286 (Mouse); 681901 (Rat)

Gene Symbol

UTF1

UniProt

Additional UTF1 Products

Product Documents for Mouse UTF1 Alexa Fluor® 350‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse UTF1 Alexa Fluor® 350‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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