Mouse VEGF R2/Flk-1 Quantikine ELISA Kit

(11 citations)   
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    0.049 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse sVEGF R2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse soluble VEGF R2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R2 in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R2.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.


The recovery of mouse sVEGF R2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 105 100-111
Plasma (n=4) 108 89-116
Serum (n=3) 104 82-120
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse sVEGF R2 in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse VEGF R2/Flk-1 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VEGF R2/KDR/Flk-1
VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.
    • Long Name
      Vascular Endothelial Growth Factor Receptor 2
    • Entrez Gene IDs
      3791 (Human); 16542 (Mouse);
    • Alternate Names
      CD309 antigen; CD309; EC 2.7.10; EC; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGFR; VEGFR2; VEGFR-2;
    Related Research Areas

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 11
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    Sample Type
    1. Long-term continuous corticosterone treatment decreases VEGF receptor-2 expression in frontal cortex.
      Authors: Howell KR, Kutiyanawalla A, Pillai A
      PLoS ONE, 2011;6(5):e20198.
      Species: Mouse
      Sample Type: Serum
    2. Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1.
      Authors: Ruiz de Almodovar C, Coulon C, Salin PA, Knevels E, Chounlamountri N, Poesen K, Hermans K, Lambrechts D, Van Geyte K, Dhondt J, Dresselaers T, Renaud J, Aragones J, Zacchigna S, Geudens I, Gall D, Stroobants S, Mutin M, Dassonville K, Storkebaum E, Jordan BF, Eriksson U, Moons L, D'Hooge R, Haigh JJ, Belin MF, Schiffmann S, Van Hecke P, Gallez B, Vinckier S, Chedotal A, Honnorat J, Thomasset N, Carmeliet P, Meissirel C
      J. Neurosci., 2010;30(45):15052-66.
      Species: Mouse
      Sample Type: Tissue Homogenates
    3. Inhibition of choroidal neovascularization with an anti-inflammatory carotenoid astaxanthin.
      Authors: Izumi-Nagai K, Nagai N, Ohgami K, Satofuka S, Ozawa Y, Tsubota K, Ohno S, Oike Y, Ishida S
      Invest. Ophthalmol. Vis. Sci., 2008;49(4):1679-85.
      Species: Mouse
      Sample Type: Tissue Homogenates
    4. Vascular endothelial growth factor-mediated decrease in plasma soluble vascular endothelial growth factor receptor-2 levels as a surrogate biomarker for tumor growth.
      Authors: Ebos JM, Lee CR, Bogdanovic E, Alami J, Van Slyke P, Francia G, Xu P, Mutsaers AJ, Dumont DJ, Kerbel RS
      Cancer Res., 2008;68(2):521-9.
      Species: Mouse
      Sample Type: Plasma
    5. (Pro)renin receptor promotes choroidal neovascularization by activating its signal transduction and tissue renin-angiotensin system.
      Authors: Satofuka S, Ichihara A, Nagai N, Noda K, Ozawa Y, Fukamizu A, Tsubota K, Itoh H, Oike Y, Ishida S
      Am. J. Pathol., 2008;173(6):1911-8.
      Species: Mouse
      Sample Type: Tissue Homogenates
    6. An Adam15 amplification loop promotes vascular endothelial growth factor-induced ocular neovascularization.
      Authors: Xie B, Shen J, Dong A, Swaim M, Hackett SF, Wyder L, Worpenberg S, Barbieri S, Campochiaro PA
      FASEB J., 2008;22(8):2775-83.
      Species: Mouse
      Sample Type: Tissue Homogenates
    7. Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
      Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
      Proc. Natl. Acad. Sci. U.S.A., 2007;104(43):17069-74.
      Species: Mouse
      Sample Type: Plasma
    8. Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy.
      Authors: Shimojo N, Jesmin S, Zaedi S, Otsuki T, Maeda S, Yamaguchi N, Aonuma K, Hattori Y, Miyauchi T
      Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H474-81.
      Species: Rat
      Sample Type: Cell Culture Supernates
    9. Exercise training improves aging-induced downregulation of VEGF angiogenic signaling cascade in hearts.
      Authors: Iemitsu M, Maeda S, Jesmin S, Otsuki T, Miyauchi T
      Am. J. Physiol. Heart Circ. Physiol., 2006;291(3):H1290-8.
      Species: Rat
      Sample Type: Tissue Homogenates
    10. Selective suppression of pathologic, but not physiologic, retinal neovascularization by blocking the angiotensin II type 1 receptor.
      Authors: Nagai N, Noda K, Urano T, Kubota Y, Shinoda H, Koto T, Shinoda K, Inoue M, Shiomi T, Ikeda E, Tsubota K, Suda T, Oike Y, Ishida S
      Invest. Ophthalmol. Vis. Sci., 2005;46(3):1078-84.
      Species: Mouse
      Sample Type: Tissue Homogenates
    11. Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia.
      Authors: Maynard SE, Min JY, Merchan J, Lim KH, Mondal S, Stillman IE, Epstein FH, Karumanchi SA
      J. Clin. Invest., 2003;111(5):649-58.
      Species: Rat
      Sample Type: Plasma
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