Mouse Vitamin D BP Antibody

Catalog # Availability Size / Price Qty
MAB41881-100
MAB41881-SP
Mouse Vitamin D BP Antibody in ELISA
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Mouse Vitamin D BP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Vitamin D BP in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 884706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Vitamin D BP
Met1-Ser476
Accession # P21614
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB4188).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Vitamin D BP DuoSet ELISA Kit (Catalog # DY4188-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Mouse Vitamin D BP ELISA Standard Curve. Recombinant Mouse Vitamin D BP protein was serially diluted 2-fold and captured by Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB41881) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB4188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vitamin D BP

VDBP (Vitamin D binding protein; also group-specific component and GC-globulin) is a 52‑58 kDa, monomeric glycoprotein member of the ALB/AFP/VDB family of molecules. It is found in blood, urine and CSF, carries Vitamin D and its metabolites, and serves as an actin-scavenging protein. Mature mouse VDBP is 460 amino acids (aa) in length. It contains three albumin-type domains (aa 26‑476) that are accompanied by 14 intrachain disulfide bonds. There are three potential alternative splice forms. One shows a deletion of aa 346‑421, a second shows a 67 aa substitution for aa 345‑421, and a third shows a 34 aa substitution for aa 346‑423. All these variants involve the second and third albumin-like domains. Mature mouse VDBP (aa 17‑476) is 77% and 90% aa identical to human and rat VDBP, respectively.

Long Name
Vitamin D Binding Protein
Entrez Gene IDs
2638 (Human); 14473 (Mouse); 24384 (Rat)
Alternate Names
DBP; DBP/GC; DBP-MAF; DBPVDBG; EC 6.3.1.5; Gc; gc-globulin; Gc-MAF; GRD3; group-specific component (vitamin D binding protein); Group-specific component; hDBP; VDB; VDBG; VDBP; Vitamin D BP; vitamin D-binding alpha-globulin; vitamin D-binding protein

Product Datasheets

FAQs

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