Mouse Vitronectin Antibody

Catalog # Availability Size / Price Qty
MAB38751
MAB38751-SP
Mouse Vitronectin Antibody in Immunohistochemistry
1 Image
Product Details
Citations (6)
FAQs
Supplemental Products
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Mouse Vitronectin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Vitronectin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Vitronectin is observed.
Source
Monoclonal Rat IgG2A Clone # 347317
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Vitronectin
Asp20-Lys478
Accession # AAH12690
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Vitronectin in Mouse Brain. View Larger

Vitronectin in Mouse Brain. Vitronectin was detected in immersion fixed frozen sections of fetal mouse brain using 10 µg/mL Rat Anti-Mouse Vitronectin Monoclonal Antibody (Catalog # MAB38751) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vitronectin

Vitronectin is a 71 kDa secreted glycoprotein produced by the liver and tumor cells. In blood, Vitronectin is called serum spreading factor. In the extracellular matrix, its function is determined by binding partners such as PAI-1, complement factors, integrins (notably alpha v beta 3) and thrombin. The 459 amino acid (aa) mature mouse Vitronectin shows 74% aa sequence identity with human Vitronectin and contains somatomedin B-like and hemopexin-like domains, an RGD motif, a basic heparin-binding domain and sulfated tyrosines. Unbound Vitronectin is a monomer that may be cleaved to form a dimer of 65 kDa and 10 kDa components.

Entrez Gene IDs
7448 (Human); 22370 (Mouse); 507525 (Bovine)
Alternate Names
Complement S-protein; epibolin; Serum Spreading Factor; Serum-spreading factor; Somatomedin B; S-protein; V75; vitronectin (serum spreading factor, somatomedin B, complement S-protein); Vitronectin; VN; VNT; VTN

Product Datasheets

Citations for Mouse Vitronectin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Extracellular vesicle integrins act as a nexus for platelet adhesion in cerebral microvessels
    Authors: Z Bagi, Y Couch, Z Broskova, F Perez-Bald, T Yeo, S Davis, R Fischer, NR Sibson, BG Davis, DC Anthony
    Sci Rep, 2019;9(1):15847.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2018;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Hepato-entrained B220+CD11c+NK1.1+ cells regulate pre-metastatic niche formation in the�lung
    Authors: S Hiratsuka, T Tomita, T Mishima, Y Matsunaga, T Omori, S Ishibashi, S Yamaguchi, T Hosogane, H Watarai, M Omori-Miya, T Yamamoto, N Shibata, A Watanabe, H Aburatani, M Tomura, KA High, Y Maru
    EMBO Mol Med, 2018;10(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness.
    Authors: Wang X, Rodda L, Bannard O, Cyster J
    J Immunol, 2014;192(10):4601-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Age-related retinopathy in NRF2-deficient mice.
    Authors: Zhao Z, Chen Y, Wang J
    PLoS ONE, 2011;6(4):e19456.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Pericytes promote endothelial cell survival through induction of autocrine VEGF-A signaling and Bcl-w expression.
    Authors: Franco M, Roswall P, Cortez E, Hanahan D, Pietras K
    Blood, 2011;118(10):2906-17.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Cell and Tissue Staining Kits

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