Mouse WISP-1/CCN4 Antibody

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Product Details
Citations (5)
Supplemental Products

Mouse WISP-1/CCN4 Antibody Summary

Species Reactivity
Detects mouse WISP-1/CCN4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 10-30% cross-reactivity with recombinant human (rh) WISP-1 but no cross-reactivity with rhCTGF or rmNOV.
Monoclonal Rat IgG2B Clone # 214203
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse WISP-1/CCN4
Accession # O54775
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse WISP‑1/CCN4 (Catalog # 1680-WS)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WISP-1/CCN4

Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1- 6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53-120), a 66 aa von Willebrand factor type C (VWC) module (aa 121-186), a 41 aa TSP type I domain (aa 220-260) and a 75 aa, C-terminal cysteine knot motif (aa 273-347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7-9). Mature mouse WISP-1 is 98%, 84%, and 85% aa identical to rat, canine, and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11).

  1. Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
  2. Tanada, S. et al. (2001) Oncogene 20:5525.
  3. Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
  4. Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
  5. Brigstock, D.R. (2003) J. Endocrinol. 178:169.
  6. Hashimoto, Y. et al. (1998) J. Exp. Med. 187:289.
  7. SwissProt Accession # O54775.
  8. SwissProt Accession # Q99PP0.
  9. SwissProt Accession # O95388
  10. French, D.M. et al. (2004) Am. J. Pathol. 165:855.
  11. Parisi, M.S. et al. (2006) Bone 38:671.
Long Name
Wnt-1 Induced Secreted Protein 1
Entrez Gene IDs
8840 (Human); 22402 (Mouse); 102125044 (Cynomolgus Monkey)
Alternate Names
CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1

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Citations for Mouse WISP-1/CCN4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. WNT1-inducible signaling pathway protein 1 (WISP1/CCN4) stimulates melanoma invasion and metastasis by promoting the epithelial - mesenchymal transition
    Authors: W Deng, A Fernandez, SL McLaughlin, DJ Klinke
    J. Biol. Chem., 2019-02-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
    Authors: Quan Li
    Mol. Med., 2016-01-06;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. SAP domain-dependent Mkl1 signaling stimulates proliferation and cell migration by induction of a distinct gene set indicative of poor prognosis in breast cancer patients.
    Authors: Gurbuz, Irem, Ferralli, Jacqueli, Roloff, Tim, Chiquet-Ehrismann, Ruth, Asparuhova, Maria B
    Mol Cancer, 2014-02-05;13(0):22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  4. A quantitative systems approach to identify paracrine mechanisms that locally suppress immune response to Interleukin-12 in the B16 melanoma model
    Authors: Yogesh M. Kulkarni, Emily Chambers, A. J. Robert McGray, Jason S. Ware, Jonathan L. Bramson, David J. Klinke II
    Integrative Biology
  5. Mechanical Stretch Induces Epithelial-Mesenchymal Transition in Alveolar Epithelia via Hyaluronan Activation of Innate Immunity.
    Authors: Heise RL, Stober V, Cheluvaraju C, Hollingsworth JW, Garantziotis S
    J. Biol. Chem., 2011-03-11;286(20):17435-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization


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