Mouse WISP-1/CCN4 Antibody (214203)

R&D Systems | Catalog # MAB1680

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 214203
View all WISP-1/CCN4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse WISP-1/CCN4
Leu21-Asn367
Accession # O54775

Specificity

Detects mouse WISP-1/CCN4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 10-30% cross-reactivity with recombinant human (rh) WISP-1 but no cross-reactivity with rhCTGF or rmNOV.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse WISP-1/CCN4 Antibody (214203)

Detection of WISP-1/CCN4 by Flow Cytometry

Detection of WISP-1/CCN4 by Flow Cytometry

Recombinant WISP-1 reduces M2 macrophage survival, induces apoptosis, and promotes reprogramming toward an M1-like phenotype. (a) Cell viability assay of THP-1-derived M1 (M1) and M2 macrophages (M2) treated with 20-100 ng/ml mouse (rWISP-1) or human WISP-1 (hrWISP-1) for 3 days. (b) Left: Flow cytometry analysis after Annexin V-FICT/PI dual staining was employed to evaluate the apoptosis of THP-1- derived M1 and M2 macrophages after treatment with hrWISP- (20-100 ng/ml) for 3 days. Right: Apoptotic cells were quantified as the sum of the percentages of early and late stages of apoptosis. (c) qRT-PCR analysis of relative mRNA levels of M1 (Nos2, MhcII, and Il12p40) and M2 (Tgf beta 1, Il10, and Il4) markers in M2 macrophages treated with 20-100 ng/ml hrWISP-1 for 3 days. (d) ELISA of TNF-alpha, IL-1 beta, IL-4, and IL-13 in the culture supernatant of M2 macrophages treated with 20-100 ng/ml hrWISP-1 for 3 days. (e) Flow cytometry analysis of the population of CD80+ and CD206+ cells among M2 macrophages after treatment with hrWISP-1 (20-100 ng/ml) for 2 or 3 days. Mean fluorescence intensity (MFI) values (right). (f) Heatmap showing differentially expressed macrophage polarization-related genes in THP-1-derived M2 macrophages (left). Red: high expression; blue: low expression. Relative expression of selected genes from PCR array profiling of macrophage polarization markers (right). Log2 fold-change values (hrWISP-1 vs. Vehicle). THP-1-derived M2 macrophages were treated with hrWISP-1 (50 ng/ml) for 3 days. NS: not significant; *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student's t-test. The data are from one experiment representative of three independent experiments with similar results (b, e, and f left) or from three independent experiments (mean ± standard error: a, c, d; b, e and f right). Image collected and cropped by CiteAb from the following open publication (https://www.ijbs.com/v22p0995.htm), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse WISP-1/CCN4 Antibody (214203)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse WISP‑1/CCN4 (Catalog # 1680-WS)
under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: WISP-1/CCN4

Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1- 6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53-120), a 66 aa von Willebrand factor type C (VWC) module (aa 121-186), a 41 aa TSP type I domain (aa 220-260) and a 75 aa, C-terminal cysteine knot motif (aa 273-347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7-9). Mature mouse WISP-1 is 98%, 84%, and 85% aa identical to rat, canine, and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11).

References

  1. Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
  2. Tanada, S. et al. (2001) Oncogene 20:5525.
  3. Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
  4. Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
  5. Brigstock, D.R. (2003) J. Endocrinol. 178:169.
  6. Hashimoto, Y. et al. (1998) J. Exp. Med. 187:289.
  7. SwissProt Accession # O54775.
  8. SwissProt Accession # Q99PP0.
  9. SwissProt Accession # O95388
  10. French, D.M. et al. (2004) Am. J. Pathol. 165:855.
  11. Parisi, M.S. et al. (2006) Bone 38:671.

Long Name

Wnt-1 Induced Secreted Protein 1

Alternate Names

CCN4, WISP1

Entrez Gene IDs

8840 (Human); 22402 (Mouse); 102125044 (Cynomolgus Monkey)

Gene Symbol

CCN4

UniProt

O54775

Additional WISP-1/CCN4 Products

Product Documents for Mouse WISP-1/CCN4 Antibody (214203)

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Product Specific Notices for Mouse WISP-1/CCN4 Antibody (214203)

For research use only

Citations for Mouse WISP-1/CCN4 Antibody (214203)

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