Mouse Wnt-4 Antibody
Mouse Wnt-4 Antibody Summary
Ile37-Glu76, Arg222-Thr295
Accession # P22724
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-4
Wnt-4 is a 38‑42 kDa member of the Wnt family of secreted glycoproteins, which act as short-range signaling molecules via Frizzled receptors and a cascade of intracellular signals in vertebrate embryogenesis (1‑2). Mouse Wnt-4 is synthesized as a 351 amino acid (aa) precursor with a 22 aa signal sequence and a 329 aa mature chain. The mature chain contains two potential sites for N-linked glycosylation. Relative to other members of the Wnt family, Wnt-4 contains 83 conserved aa, including 21 cysteines (1). Mature mouse Wnt-4 shares 99% aa sequence identity with mature human and rat Wnt-4. Wnt-4 has been shown to play a critical role in the development of the reproductive system and in the formation of the kidneys, adrenals, pituitary gland, and mammary tissues (3‑6). In the development of the reproductive system, Wnt-4 expression is down-regulated in the developing gonad after E11.5, although it persists in the developing ovary (2, 6). Targeted deletion of Wnt-4 results in masculinization of XX mice, with rudimentary development of the masculine internal (Wolffian) ducts and degeneration of the female (Mullerian) reproductive tract (2, 6). In addition to its involvement in urogenital development, Wnt-4 is also expressed in the perichondrium of the long bones (7), and promotes osteoblast differentiation (8). Wnt-4 may also be associated with abnormal proliferation in human breast tissue (9). In humans, mutations in Wnt-4 are the cause of SERKAL syndrome, a syndrome consisting of female to male sex reversal, renal, adrenal, and lung dysgenesis, and developmental defects (3), and Rokitansky-Kuster-Hauser syndrome, which is characterized by utero-vaginal atresia in otherwise phenotypically normal females with normal 46, XX karyotype (10).
- Gavin, B.J. et al. (1990) Genes Dev. 4:2319.
- Jordan, B.K. et al. (2001) Am. J. Hum. Genet. 68:1102.
- Mandel, H. et al. (2008) Am. J. Hum. Genet. 82:39.
- Bernard, P. and V.R. Harley (2007) Int. J. Biochem. Cell Biol. 39:31.
- Kuulasmaa, T. et al. (2008) Horm. Metab. Res. 40:668.
- Vainio, S. et al. (1999) Nature 397:405.
- Gao, X. (2004) Genes Dev. 18:2404.
- Chang, J. et al. (2007) J. Biol. Chem. 282:30938.
- Huguet, E.L. et al. (1994) Cancer Res. 54:2615.
- Philibert, P. et al. (2008) J. Clin. Endocrinol. Metab. 93:895.
Product Datasheets
Citations for Mouse Wnt-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Wnt4 is heterogeneously activated in maturing beta-cells to control calcium signaling, metabolism and function
Authors: K Katsumoto, S Yennek, C Chen, LFD Silva, S Traikov, D Sever, A Azad, J Shan, S Vainio, N Ninov, S Speier, A Grapin-Bot
Nature Communications, 2022;13(1):6255.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
GLI1-expressing mesenchymal cells form the essential Wnt-secreting niche for colon stem cells
Authors: B Degirmenci, T Valenta, S Dimitrieva, G Hausmann, K Basler
Nature, 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism
Authors: LY Hung, D Sen, TK Oniskey, J Katzen, NA Cohen, AE Vaughan, W Nieves, A Urisman, MF Beers, MF Krummel, DR Herbert
Mucosal Immunol, 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Identification of proliferative and mature ?-cells in the islets of Langerhans
Nature, 2016;535(7612):430-4.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation
Authors: Bruce F Kimler
J. Endocrinol., 2016;229(2):197-207.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Lgr5 marks stem/progenitor cells in ovary and tubal epithelia.
Authors: Ng A, Tan S, Singh G, Rizk P, Swathi Y, Tan T, Huang R, Leushacke M, Barker N
Nat Cell Biol, 2014;16(8):745-57.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dickkopf-1 is a master regulator of joint remodeling.
Authors: Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
Nat. Med., 2007;13(2):156-63.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
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