Mouse Wnt-9b Antibody

Catalog # Availability Size / Price Qty
AF3669
AF3669-SP
Wnt‑9b in Mouse Embryo.
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Product Details
Citations (3)
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Mouse Wnt-9b Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Wnt-9b in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Wnt‑2, rhWnt-9a, recombinant mouse (rm) Wnt‑1, rmWnt-2b, rmWnt-3a, rmWnt-4, rmWnt‑5a, rmWnt-5b, rmWnt-11, and rmWnt-16 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Wnt‑9b
Ala46-Glu90, Lys228-Tyr298
Accession # O35468
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Wnt‑9b (Catalog # 3669-WN)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Wnt-9b antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Wnt‑9b in Mouse Embryo. Wnt-9b was detected in imm-ersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Wnt-9b Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF3669) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to the retina. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-9b

Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts mainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336 aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shares 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to direct mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5‑7). Induction is dependent on beta -catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations of Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4).

References
  1. http://www.stanford.edu/group/nusselab/cgi-bin/wnt/.
  2. Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
  3. Kirikoshi, H. and  M. Katoh (2002) Int. J. Mol. Med. 9:135.
  4. Qian, J. et al. (2003) Genomics 81:34.
  5. Carroll, T. J. et al. (2005) Dev. Cell 9:283.
  6. Merkel, C. E. et al. (2007) Pediatr. Nephrol. 22:1825.
  7. Park, J-S. et al. (2007) Development 134:2533.
  8. Lan, Y. et al. (2006) Dev. Dyn. 235:1448.
  9. Juriloff, D. M. and  M. J. Harris (2008) Birth Defects Res. A Clin. Mol. Teratol. 82:63.
Long Name
Wingless-type MMTV Integration Site Family, Member 9b
Entrez Gene IDs
7484 (Human); 22412 (Mouse); 303586 (Rat)
Alternate Names
Protein Wnt-14b; wingless-type MMTV integration site family, member 15; wingless-type MMTV integration site family, member 9B; Wnt-14b; WNT14Bprotein Wnt-9b; Wnt-15; WNT15Protein Wnt-15; Wnt9b; Wnt-9b

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Citations for Mouse Wnt-9b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Exploring the Role of Hypoxia-Inducible Carbonic Anhydrase IX (CAIX) in Circulating Tumor Cells (CTCs) of Breast Cancer
    Authors: JD Twomey, B Zhang
    Biomedicines, 2023-03-17;11(3):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions
    Authors: P Nowialis, K Lopusna, J Opavska, SL Haney, A Abraham, P Sheng, A Riva, A Natarajan, O Guryanova, M Simpson, R Hlady, M Xie, R Opavsky
    Nat Commun, 2019-09-26;10(1):4374.
    Species: Mouse
    Sample Types: Tissue Lystates
    Applications: Western Blot
  3. Wnt signaling in the pathogenesis of human HIV-associated pain syndromes.
    Authors: Shi, Yuqiang, Shu, Jianhong, Gelman, Benjamin, Lisinicchia, Joshua G, Tang, Shao-Jun
    J Neuroimmune Pharmacol, 2013-06-05;8(4):956-64.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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