Mouse Wnt-9b Antibody Summary
Ala46-Glu90, Lys228-Tyr298
Accession # O35468
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Wnt‑9b in Mouse Embryo. Wnt-9b was detected in imm-ersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Wnt-9b Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF3669) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to the retina. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of Wnt-9b by Western Blot Identification of a panel of breast cancer cell lines with emergent anoikis-resistant cancer stem cell populations. (A) A panel of seven breast cancer anoikis-resistant cell lines was evaluated for cancer stem cell markers CD44, CD24, and Sox2 following culturing in either a monolayer (day 0) or non-adherent suspension condition for up to seven days using immunoblotting. Total protein expression for CD44 and Sox2 was increased over the seven days of culturing relative to the monolayer condition. CD24 was decreased in the MCF7 and T47D cell lines, and low expression was maintained in the MB-231 cell line. Immunoblot images are representative of 3 biological replicates. (B) The surface expression of CD44 and CD24 was analyzed using multispectral imaging flow cytometry (MIFC), and the CD44+CD24− population was quantified from the bivariate graph (C). The data shown are the percentages of CD44+CD24− cells out of the total population. Bivariate charts were gated on the isotype controls for each marker. (n = 3, * p < 0.05 relative to the monolayer culture (D0)). (D) MIFC images were taken of individual cells at each time point, and representative images for MB-231, MCF7, and T47D cells at days 0 (D0) and 7 (D7) of suspension culturing are shown. Assigned cell number of population is shown on the brightfield images. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2227-9059/11/3/934), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-9b
Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts mainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336 aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shares 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to direct mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5‑7). Induction is dependent on beta -catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations of Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4).
- http://www.stanford.edu/group/nusselab/cgi-bin/wnt/.
- Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
- Kirikoshi, H. and M. Katoh (2002) Int. J. Mol. Med. 9:135.
- Qian, J. et al. (2003) Genomics 81:34.
- Carroll, T. J. et al. (2005) Dev. Cell 9:283.
- Merkel, C. E. et al. (2007) Pediatr. Nephrol. 22:1825.
- Park, J-S. et al. (2007) Development 134:2533.
- Lan, Y. et al. (2006) Dev. Dyn. 235:1448.
- Juriloff, D. M. and M. J. Harris (2008) Birth Defects Res. A Clin. Mol. Teratol. 82:63.
Product Datasheets
Citations for Mouse Wnt-9b Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Exploring the Role of Hypoxia-Inducible Carbonic Anhydrase IX (CAIX) in Circulating Tumor Cells (CTCs) of Breast Cancer
Authors: JD Twomey, B Zhang
Biomedicines, 2023-03-17;11(3):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions
Authors: P Nowialis, K Lopusna, J Opavska, SL Haney, A Abraham, P Sheng, A Riva, A Natarajan, O Guryanova, M Simpson, R Hlady, M Xie, R Opavsky
Nat Commun, 2019-09-26;10(1):4374.
Species: Mouse
Sample Types: Tissue Lystates
Applications: Western Blot -
Wnt signaling in the pathogenesis of human HIV-associated pain syndromes.
Authors: Shi, Yuqiang, Shu, Jianhong, Gelman, Benjamin, Lisinicchia, Joshua G, Tang, Shao-Jun
J Neuroimmune Pharmacol, 2013-06-05;8(4):956-64.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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