Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Neutralization, Flow Cytometry, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 80222
View all XCL1/Lymphotactin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse XCL1/Lymphotactin
Val22-Gly114
Accession # AAA56752

Specificity

Detects mouse XCL1/Lymphotactin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human XCL1/Lymphotactin is observed and no cross-reactivity with recombinant viral (rv) CMV UL147 is observed. In Western blots, 75% cross‑reactivity with rvCMV UL147 is observed and no cross-reactivity with recombinant human CCL1-3, 5, 7, 8, 11, 13-25, 28, recombinant mouse CCL1-7, 9, 11, 12, 15, 19, 21, 22, 24, 25, 27 or 28 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse XCL1/Lymphotactin Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)

Mouse XCL1/Lymphotactin Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse XCL1/Lymphotactin Biotinylated Antibody (Catalog # BAF486)
  • Standard: Recombinant Mouse XCL1/Lymphotactin Protein (Catalog # 486-LT)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: XCL1/Lymphotactin

Mouse XCL1, also known as Lymphotactin, and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse XCL1 encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of XCL1 is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for XCL1 has been mapped to chromosome 1 in both human and mouse. Recombinant mouse XCL1 has been shown to have chemotactic activity for lymphocytes, NK cells and mouse splenocytes.

References

  1. Kelner, G. et al. (1994) Science 266:1395.
  2. Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.

Long Name

Chemokine [C Motif] Ligand 1

Alternate Names

ATAC, Lymphotactin, SCM-1 alpha

Entrez Gene IDs

6375 (Human); 16963 (Mouse)

Gene Symbol

XCL1

UniProt

AAA56752

Additional XCL1/Lymphotactin Products

Product Documents for Mouse XCL1/Lymphotactin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse XCL1/Lymphotactin Antibody

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Mouse XCL1/Lymphotactin Antibody

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