Mouse XCL1/Lymphotactin Antibody

Catalog # Availability Size / Price Qty
MAB486
MAB486-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Mouse XCL1/Lymphotactin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse XCL1/Lymphotactin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human XCL1/Lymphotactin is observed and no cross-reactivity with recombinant viral (rv) CMV UL147 is observed. In Western blots, 75% cross‑reactivity with rvCMV UL147 is observed and no cross-reactivity with recombinant human CCL1-3, 5, 7, 8, 11, 13-25, 28, recombinant mouse CCL1-7, 9, 11, 12, 15, 19, 21, 22, 24, 25, 27 or 28 is observed.
Source
Monoclonal Rat IgG2A Clone # 80222
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse XCL1/Lymphotactin
Val22-Gly114
Accession # AAA56752
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)

Mouse XCL1/Lymphotactin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse XCL1/Lymphotactin Biotinylated Antibody (Catalog # BAF486)

Standard: Recombinant Mouse XCL1/Lymphotactin Protein (Catalog # 486-LT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XCL1/Lymphotactin

Mouse XCL1, also known as Lymphotactin, and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse XCL1 encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of XCL1 is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for XCL1 has been mapped to chromosome 1 in both human and mouse. Recombinant mouse XCL1 has been shown to have chemotactic activity for lymphocytes, NK cells and mouse splenocytes.

References
  1. Kelner, G. et al. (1994) Science 266:1395.
  2. Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.
Long Name
Chemokine [C Motif] Ligand 1
Entrez Gene IDs
6375 (Human); 16963 (Mouse)
Alternate Names
ATAC; ATACSmall-inducible cytokine C1; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; LPTNSCM-1-alpha; LTNXC chemokine ligand 1; Lymphotactin; lymphotaxin; SCM-1 alpha; SCM-1; SCM1A; SCM-1a; SCYC1SCM1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); XCL1

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Citations for Mouse XCL1/Lymphotactin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. XCR1+ DCs are critical for T�cell-mediated immunotherapy of chronic viral infections
    Authors: E Domenjo-Vi, V Casella, R Iwabuchi, E Fossum, M Pedragosa, Q Castellví, P Cebollada, T Kaisho, K Terahara, G Bocharov, J Argilaguet, A Meyerhans
    Oncogene, 2023-02-14;42(2):112123.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. XCR1+ type 1 conventional dendritic cells drive liver pathology in non-alcoholic steatohepatitis
    Authors: A Deczkowska, E David, P Ramadori, D Pfister, M Safran, B At The, A Giladi, DA Jaitin, O Barboy, M Cohen, I Yofe, C Gur, S Shlomi-Lou, S Henri, Y Suhail, M Qiu, S Kam, H Hermon, E Lahat, G Ben Yakov, O Cohen-Ezra, Y Davidov, M Likhter, D Goitein, S Roth, A Weber, B Malissen, A Weiner, Z Ben-Ari, M Heikenwäld, E Elinav, I Amit
    Nature Medicine, 2021-05-20;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control
    Authors: JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou
    Cell, 2018-02-08;172(5):1022-1037.e14.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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