MPG Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82787

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MPG Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (84%). Human reactivity reported in scientific literature (PMID: 23290262).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MPG Antibody - BSA Free

Western Blot: MPG Antibody [NBP1-82787]

Western Blot: MPG Antibody [NBP1-82787]

Western Blot: MPG Antibody [NBP1-82787] - Analysis in human cell lines MCF-7 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: MPG Antibody [NBP1-82787]

Immunocytochemistry/ Immunofluorescence: MPG Antibody [NBP1-82787]

Immunocytochemistry/Immunofluorescence: MPG Antibody [NBP1-82787] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MPG Antibody [NBP1-82787]

Immunohistochemistry-Paraffin: MPG Antibody [NBP1-82787]

Immunohistochemistry-Paraffin: MPG Antibody [NBP1-82787] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.

Applications for MPG Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
WB, ICC/IF reported in scientific literature (PMID: 23290262). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 2 reviews rated 3 using NBP1-82787 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MPG

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions

Alternate Names

3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on16p, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, AAG, ADPG, anpg, APNG, CRA36.1, CRA36.1 (3-methyl-adenine DNA glycosylase), DNA-3-methyladenine glycosylase, EC 3.2.2.21, MDGalkyladenine DNA glycosylase, Mid1, N-methylpurine-DNA glycosylase, MPG, N-methylpurine-DNA glycosylasePIG16, PIG11, proliferation-inducing protein 11, proliferation-inducing protein 16

Gene Symbol

MPG

Additional MPG Products

Product Documents for MPG Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MPG Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MPG Antibody - BSA Free

Customer Reviews for MPG Antibody - BSA Free (2)

3 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • MPG Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: HEK293
    Species: Human
    Verified Customer | Posted 11/16/2018
    MPG in HEK293 Cells
    4ug/mL overnight at 4C in 1X PBS/1% BSA/0.3% Triton™ X-100. Nuclear signal not as strong and seemed more diffuse when compared to staining done with another MPG antibody done at the same time.
    MPG Antibody - BSA Free NBP1-82787
  • MPG Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lymphocytes
    Species: Human
    Verified Customer | Posted 09/20/2018
    MPG in Human Lymphocytes
    30ug of Lymphocyte whole cell lysate. Antibody used at 0.4ug/mL in 5% milk-TBS/T. 5 minute exposure.
    MPG Antibody - BSA Free NBP1-82787

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