MPST Antibody - BSA Free

Immunohistochemistry-Paraffin: MPST Antibody [NBP1-82617]

Immunohistochemistry-Paraffin: MPST Antibody [NBP1-82617] - Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.

Simple Western: MPST Antibody [NBP1-82617]

Simple Western: MPST Antibody [NBP1-82617] - Simple Western lane view shows a specific band for MPST in 0.2 mg/ml of Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: MPST Antibody [NBP1-82617]

Simple Western: MPST Antibody [NBP1-82617] - Electropherogram image(s) of corresponding Simple Western lane view. MPST antibody was used at 1:20 dilution on Liver lysate(s).

Simple Western: MPST Antibody [NBP1-82617]

Simple Western: MPST Antibody [NBP1-82617] - Electropherogram image(s) of corresponding Simple Western lane view. MPST antibody was used at 1:20 dilution on RT-4 lysate(s).

Immunohistochemistry: MPST Antibody - BSA Free [NBP1-82617] -

3-MST deficiency exacerbates joint calcification and cartilage damage in experimental OA. a Representative immunohistochemical analysis of 3-MST expression in the knee section from sham-operated and MNX WT mice. A knee section from a sham-operated 3-Mst KO mouse was used to prove the specificity of the 3-MST antibody. Scale bars 150 μm. b Representative micro-CT scan images of WT and 3-MST KO MNX murine knee joints two months after surgery. White arrows show calcified periarticular deposits in MNX WT knees and their exacerbation in 3-MST KO mice. Graphs show CTAnalyzer quantitative analysis of new formation volume (mm3) and new formation crystal content (μg) in WT and 3-MST KO MNX knees. c Representative histologies of WT and 3-MST KO MNX knees, stained with Safranin-O. Black arrows show increased cartilage degradation in 3-MST KO mice. Scale bars 150 μm. Graphs show femoral scoring of cartilage damage and Safranin-O loss, accordingly to OARSI method. d Thiosulfate measurement in the serum of WT and 3-MST KO mice. Mice number WT n = 8, 3-MST KO n = 8 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32183900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: MPST Antibody - BSA Free [NBP1-82617] -

Cartilage 3-MST expression is negatively correlated with OA severity and chondrocyte calcification and downmodulated by HA crystals. a 3-MST immunohistochemical staining in cartilage explants from end-stage osteoarthritis patients and consecutive sections stained with von Kossa/Safranin-O staining for calcium-containing crystals. For each staining, one representative picture from one out of 14 patients is shown. Scale bars 200 μm. The graph shows the correlation between the % of 3-MST-positive cells and the % of calcified cartilage in the different patients. n = 14 patients. b Immunohistochemical staining of 3-MST in cartilage from individuals with low, medium, and high stage osteoarthritis and von Kossa/Safranin-O staining for calcium-containing crystals in consecutive sections. Pictures from one representative patient out of 5 patients per group are shown. Scale bars 200 μm. The graphs show the % of 3-MST-positive cells and the % of calcified cartilage. Three fields were counted per patient and the mean plotted in the graph. n = 14 patients. c 3-MST immunohistochemistry of human cartilage explants stimulated 24 h with HA crystals (HA 500 μg/ml) or not (Nt). Scale bars 200 μm. The graph shows the % of 3-MST-positive cells in Nt vs HA-treated explants in each patient. Lines connect the Nt condition and the HA condition for each patient. n = 4 patients Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32183900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: MPST Antibody - BSA Free [NBP1-82617] -

Cartilage 3-MST expression is negatively correlated with OA severity and chondrocyte calcification and downmodulated by HA crystals. a 3-MST immunohistochemical staining in cartilage explants from end-stage osteoarthritis patients and consecutive sections stained with von Kossa/Safranin-O staining for calcium-containing crystals. For each staining, one representative picture from one out of 14 patients is shown. Scale bars 200 μm. The graph shows the correlation between the % of 3-MST-positive cells and the % of calcified cartilage in the different patients. n = 14 patients. b Immunohistochemical staining of 3-MST in cartilage from individuals with low, medium, and high stage osteoarthritis and von Kossa/Safranin-O staining for calcium-containing crystals in consecutive sections. Pictures from one representative patient out of 5 patients per group are shown. Scale bars 200 μm. The graphs show the % of 3-MST-positive cells and the % of calcified cartilage. Three fields were counted per patient and the mean plotted in the graph. n = 14 patients. c 3-MST immunohistochemistry of human cartilage explants stimulated 24 h with HA crystals (HA 500 μg/ml) or not (Nt). Scale bars 200 μm. The graph shows the % of 3-MST-positive cells in Nt vs HA-treated explants in each patient. Lines connect the Nt condition and the HA condition for each patient. n = 4 patients Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32183900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.