MRG15 Antibody

Novus Biologicals | Catalog # NB100-1307

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MRG15 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence APKQDPKPKFQEG-C corresponding to N-Terminus according to NP_006782.1, NP_996670.1.

Specificity

This antibody is expected to recognise isoform 1 (NP_006782.1) and isoform 2 (NP_996670.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MRG15 Antibody

Western Blot: MRG15 Antibody [NB100-1307]

Western Blot: MRG15 Antibody [NB100-1307]

Western Blot: MRG15 Antibody [NB100-1307] - staining of NIH3T3 (A) and HepG2 (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot: MRG15 Antibody [NB100-1307]

Western Blot: MRG15 Antibody [NB100-1307]

Western Blot: MRG15 Antibody [NB100-1307] - staining of Mouse Liver (A), Rat Liver (B) and Pig Liver (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
MRG15 Antibody

Immunohistochemistry-Paraffin: MRG15 Antibody [NB100-1307] -

Immunohistochemistry-Paraffin: MRG15 Antibody [NB100-1307] - (5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
MRG15 Antibody

Immunohistochemistry-Paraffin: MRG15 Antibody [NB100-1307] -

Immunohistochemistry-Paraffin: MRG15 Antibody [NB100-1307] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
MRG15 Antibody

Immunohistochemistry: Goat Polyclonal MRG15 Antibody [NB100-1307] -

Immunohistochemistry: Goat Polyclonal MRG15 Antibody [NB100-1307] - (5µg/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
MRG15 Antibody

Control: Goat Polyclonal MRG15 Antibody [NB100-1307] -

Control: Goat Polyclonal MRG15 Antibody [NB100-1307] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.

Applications for MRG15 Antibody

Application
Recommended Usage

Immunohistochemistry

4-6 ug/ml

Immunohistochemistry-Paraffin

4-6 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1-0.3 ug/ml
Application Notes
Western blot: Approx 37kDa band observed in nuclear lysates of cell lines HepG2 and NIH3T3 and approx 40kDa band observed in Mouse, Rat and Pig Liver lysates (calculated MW of 37.2kDa according to Human NP_006782.1, Mouse NP_077751.1, Rat NP_001011999.1 and Pig XP_001927776.1 and 41.5kDa according to Human NP_996670.1, Mouse NP_001034236.1 and Pig XP_001927789.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MRG15

MRG15 is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and

Alternate Names

Eaf3, Esa1p-associated factor 3 homolog, HsT17725, MEAF3, MGC10631, MORF-related gene on chromosome 15, MORFRG15, mortality factor 4 like 1, mortality factor 4-like protein 1, MRG15MORF-related gene 15 protein, Protein MSL3-1, S863-6, Transcription factor-like protein MRG15

Entrez Gene IDs

10933 (Human); 21761 (Mouse)

Gene Symbol

MORF4L1

UniProt

Q9UBU8 (Human)

Additional MRG15 Products

Product Documents for MRG15 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MRG15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MRG15 Antibody

There are currently no reviews for this product. Be the first to review MRG15 Antibody and earn rewards!

Have you used MRG15 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies