MST1/STK4 Antibody (4F4) - Azide and BSA Free

Novus Biologicals | Catalog # H00006789-M02

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F4

Format

Azide and BSA Free
View all MST1/STK4 Primary Antibodies »

Product Specifications

Immunogen

MST1/STK4 (NP_006273, 391 a.a. ~ 485 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AKPSFLEYFEQKEKENQINSFGKSVPGPLKNSSDWKIPQDGDYEFLKSWTVEDLQKRLLALDPMMEQEIEEIRQKYQSKRQPILDAIEAKKRRQQ

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MST1/STK4 Antibody (4F4) - Azide and BSA Free

Western Blot: STK4 Antibody (4F4) [H00006789-M02]

Western Blot: STK4 Antibody (4F4) [H00006789-M02]

Western Blot: STK4 Antibody (4F4) [H00006789-M02] - STK4 monoclonal antibody (M02), clone 4F4. Analysis of STK4 expression in Raw 264.7.
Immunocytochemistry/ Immunofluorescence: STK4 Antibody (4F4) [H00006789-M02]

Immunocytochemistry/ Immunofluorescence: STK4 Antibody (4F4) [H00006789-M02]

Immunocytochemistry/Immunofluorescence: STK4 Antibody (4F4) [H00006789-M02] - Analysis of monoclonal antibody to STK4 on HeLa cell. Antibody concentration 25 ug/ml.
Western Blot: STK4 Antibody (4F4) [H00006789-M02]

Western Blot: STK4 Antibody (4F4) [H00006789-M02]

Western Blot: STK4 Antibody (4F4) [H00006789-M02] - STK4 monoclonal antibody (M02), clone 4F4 Analysis of STK4 expression in HeLa.
Western Blot: STK4 Antibody (4F4) [H00006789-M02]

Western Blot: STK4 Antibody (4F4) [H00006789-M02]

Western Blot: STK4 Antibody (4F4) [H00006789-M02] - STK4 monoclonal antibody (M02), clone 4F4. Analysis of STK4 expression in PC-12.
Western Blot: STK4 Antibody (4F4) [H00006789-M02]

Western Blot: STK4 Antibody (4F4) [H00006789-M02]

Western Blot: STK4 Antibody (4F4) [H00006789-M02] - STK4 monoclonal antibody (M02), clone 4F4. Analysis of STK4 expression in NIH/3T3.
Proximity Ligation Assay: STK4 Antibody (4F4) [H00006789-M02]

Proximity Ligation Assay: STK4 Antibody (4F4) [H00006789-M02]

Proximity Ligation Assay: STK4 Antibody (4F4) [H00006789-M02] - Analysis of protein-protein interactions between TP53 and STK4. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-STK4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
ELISA: STK4 Antibody (4F4) [H00006789-M02]

ELISA: STK4 Antibody (4F4) [H00006789-M02]

ELISA: STK4 Antibody (4F4) [H00006789-M02] - Detection limit for recombinant GST tagged STK4 is approximately 0.1ng/ml as a capture antibody.

Applications for MST1/STK4 Antibody (4F4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MST1/STK4

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.

Long Name

Mammalian STE20-like Protein Kinase 1/Serine/Threonine Protein Kinase 4

Alternate Names

KRS2, STK4, YSK3

Entrez Gene IDs

6789 (Human)

Gene Symbol

STK4

OMIM

604965 (Human)

UniProt

NP_006273 (Human)

Additional MST1/STK4 Products

Product Documents for MST1/STK4 Antibody (4F4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MST1/STK4 Antibody (4F4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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