MST2/STK3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32313

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Chicken (98%), Rat (99%), Rhesus Macaque (99%), Xenopus (98%), Zebrafish (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MST2/STK3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MST2/STK3. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (98%).

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MST2/STK3 Antibody - BSA Free

Western Blot: MST2/STK3 Antibody [NBP1-32313]

Western Blot: MST2/STK3 Antibody [NBP1-32313]

Western Blot: MST2/STK3 Antibody [NBP1-32313] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE diluted at 1:5000
Immunocytochemistry/ Immunofluorescence: MST2/STK3 Antibody [NBP1-32313]

Immunocytochemistry/ Immunofluorescence: MST2/STK3 Antibody [NBP1-32313]

Immunocytochemistry/Immunofluorescence: MST2/STK3 Antibody [NBP1-32313] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: MST2/STK3 Antibody [NBP1-32313]

Immunohistochemistry-Paraffin: MST2/STK3 Antibody [NBP1-32313]

Immunohistochemistry-Paraffin: MST2/STK3 Antibody [NBP1-32313] - Paraffin-embedded GASTRIC CA, using antibody at 1:100 dilution.
Western Blot: MST2/STK3 Antibody [NBP1-32313]

Western Blot: MST2/STK3 Antibody [NBP1-32313]

Western Blot: MST2/STK3 Antibody [NBP1-32313] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: STK3 transfected 293T lysates 7. 5% SDS PAGE; antibody diluted at 1:5000.

Applications for MST2/STK3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MST2/STK3

Mammalian Sterile 20-Like 2 (Mst2) is most similar to the previously identified Mst1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in-vitro kinase assay indicates that Mst2 can phosphorylate an exogenous substrate, as well as itself, and phospho-amino-acid analysis indicates that it is a serine/threonine protein kinase (1) Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2. Results indicate that Mst2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway (2). When stably expressed in HeLa cells, Mst highly sensitizes the cells to death receptor-mediated apoptosis by accelerating caspase-3 activation. These findings suggest that Mst1 and Mst2 play a role in apoptosis both upstream and downstream of caspase activation (3).

Alternate Names

KRS1, Mess1, MST3, STK3

Gene Symbol

STK3

Additional MST2/STK3 Products

Product Documents for MST2/STK3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MST2/STK3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MST2/STK3 Antibody - BSA Free

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  • Q: Do you have any information on how this antibody was generated?

    A: The immunogen used to generate this antibody does include aa 118-228; however, the immunogen is 234 aa in length and does contain regions outside of that range. Based on a blast search, this immunogen shares a 97% homology with STK4.

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