MST2/STK3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89586

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MST2/STK3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MST2/STK3 Antibody - BSA Free

MST2/STK3 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: MST2/STK3 Antibody - BSA Free [NBP1-89586]

Immunocytochemistry/ Immunofluorescence: MST2/STK3 Antibody - BSA Free [NBP1-89586]

Staining of human cell line U-2 OS shows localization to rods & rings.
Immunohistochemistry-Paraffin: MST2/STK3 Antibody [NBP1-89586]

Immunohistochemistry-Paraffin: MST2/STK3 Antibody [NBP1-89586]

Immunohistochemistry-Paraffin: MST2/STK3 Antibody [NBP1-89586] - Staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.
MST2/STK3 Antibody - BSA Free Western Blot: MST2/STK3 Antibody - BSA Free [NBP1-89586]

Western Blot: MST2/STK3 Antibody - BSA Free [NBP1-89586]

Analysis in human cell line HEK 293.
MST2/STK3 Antibody - BSA Free Western Blot: MST2/STK3 Antibody - BSA Free [NBP1-89586]

Western Blot: MST2/STK3 Antibody - BSA Free [NBP1-89586]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for MST2/STK3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MST2/STK3

Mammalian Sterile 20-Like 2 (Mst2) is most similar to the previously identified Mst1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in-vitro kinase assay indicates that Mst2 can phosphorylate an exogenous substrate, as well as itself, and phospho-amino-acid analysis indicates that it is a serine/threonine protein kinase (1) Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2. Results indicate that Mst2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway (2). When stably expressed in HeLa cells, Mst highly sensitizes the cells to death receptor-mediated apoptosis by accelerating caspase-3 activation. These findings suggest that Mst1 and Mst2 play a role in apoptosis both upstream and downstream of caspase activation (3).

Alternate Names

KRS1, Mess1, MST3, STK3

Gene Symbol

STK3

Additional MST2/STK3 Products

Product Documents for MST2/STK3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MST2/STK3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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