MTHFD1L Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-57852

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MTHFD1L Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: HGSLEAALQCLFQRKGSMTMSIQWKTRQLQSKLHEADIVVLGSPKPEEIPLTWIQPGTTVLNCSHDFLSGKVGCGS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MTHFD1L Antibody - BSA Free

Western Blot: MTHFD1L Antibody [NBP2-57852]

Western Blot: MTHFD1L Antibody [NBP2-57852]

Western Blot: MTHFD1L Antibody [NBP2-57852] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: MTHFD1L Antibody [NBP2-57852]

Immunocytochemistry/ Immunofluorescence: MTHFD1L Antibody [NBP2-57852]

Immunocytochemistry/Immunofluorescence: MTHFD1L Antibody [NBP2-57852] - Staining of human cell line MCF7 shows localization to mitochondria. Antibody staining is shown in green.
Simple Western: MTHFD1L Antibody [NBP2-57852]

Simple Western: MTHFD1L Antibody [NBP2-57852]

Simple Western: MTHFD1L Antibody [NBP2-57852] - Human U251 glioma cell line; whole cell lysate. Antibody at 1:250, 0.08 ug/uL total protein. Simple Western image submitted by a verified customer review.

Applications for MTHFD1L Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Simple Western

1:250

Western Blot

0.04 - 0.4 ug/mL
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. This MTHFD1L Antibody is validated for Simple Western from a verified customer review.
See Simple Western Antibody Database for Simple Western validation: Tested in Human U251 glioma cell line, whole cell lysate, separated by Size, antibody dilution of 1:250

Reviewed Applications

Read 1 review rated 4 using NBP2-57852 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MTHFD1L

MTHFD1L, also known as Monofunctional C1-tetrahydrofolate synthase, mitochondrial, consists of 2 a 978 amino acid isoform1 that is 106 kDa and a 275 amino acid isoform 2 that is 30 kDa; has mitochondrion subcellular location and highest expression found in placenta, thymus and brain; it is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion which is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine, and also may provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. Current research is being performed on this protein involvement in cleft lip/palate, neural tube defect, homocysteine, coronary heart disease, chronic myeloid leukemia, Down syndrome, colon adenocarcinoma, nephropathy, Alzheimer's disease, dementia, colorectal cancer, leukemia, and atherosclerosis. This protein plays role in One carbon pool by folate and Metabolic pathways where it interacts with CASP4, MAGED1, WRAP73, WDR8, MAP1LC3A, and plus more than 40 other proteins.

Alternate Names

10-formyl-THF synthetase, dJ292B18.2, DKFZp586G1517, EC 6.3.4.3, FLJ21145, Formyltetrahydrofolate synthetase, formyltetrahydrofolate synthetase domain containing 1, FTHFSDC1, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like, mitochondrial C1-tetrahydrofolate synthase, mitochondrial C1-tetrahydrofolate synthetase, monofunctional C1-tetrahydrofolate synthase, mitochondrial, MTC1THFS

Gene Symbol

MTHFD1L

Additional MTHFD1L Products

Product Documents for MTHFD1L Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MTHFD1L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MTHFD1L Antibody - BSA Free (1)

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  • MTHFD1L Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: U251 glioma cell line; whole cell lysate
    Species: Human
    Verified Customer | Posted 02/03/2020
    matériel: 1x25capillaires 12-230kDa Anticorps 1/250 Protéine : 0.08µg/µL
    MTHFD1L Antibody - BSA Free NBP2-57852
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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