MUC2 Antibody (CCP58)

Novus Biologicals | Catalog # NBP2-25221

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CCP58
View all MUC2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of 29 amino acids, KYPTTTPISTTTMVTPTPTPTGTQTPTTT from MUC2 protein, coupled to KLH. (Uniprot: Q02817)

Epitope

GTQTP(GlyThrGlnThrPro)

Localization

Cytoplasmic and Cell surface

Specificity

Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This monoclonal antibody shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Its epitope has been defined as GTQTP(GlyThrGlnThrPro). Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

520 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33157)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for MUC2 Antibody (CCP58)

Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221]

Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221]

Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-paraffin normal human intestine (20X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.
Western Blot: MUC2 Antibody (CCP58) [NBP2-25221]

Western Blot: MUC2 Antibody (CCP58) [NBP2-25221]

Western Blot: MUC2 Antibody (CCP58) [NBP2-25221] - MDA MB 231 cells and HCC827 human non-small cell lung cancer cell line lysates. Image from verified customer review.
Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221]

Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221]

Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC2 Antibody (CCP58).
Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221]

Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221]

Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-fixed, paraffin-embedded normal human intestine (10X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.
MUC2 Antibody (CCP58)

Western Blot: MUC2 Antibody (CCP58) [NBP2-25221] -

(A) Western blot of full-length MUC2 protein (540 kDa) and beta -actin (43 kDa) in different cell lines LS-174T CTRL, LS-174T MUC2 K.O., HT-29 CTRL, and HT-29 MUC2 K.O. grown in 2D (N = 3 biological replicates). (B) Western blot of full-length MUC2 protein (540 kDa) and beta -actin (43 kDa) in different cell lines LS-174T CTRL, LS-174T MUC2 K.O., HT-29 CTRL, and HT-29 MUC2 K.O. grown in 2D (N = 3 biological replicates). (C) Representative images of spheroids of HT-29 and LS-174T CTRL and MUC2 K.O. immunostaining with mouse anti-hMUC2 antibody (green) and counterstained with DAPI. Images acquired on confocal LS780. Scale bar, 100 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39926604), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MUC2 Antibody (CCP58)

Western Blot: MUC2 Antibody (CCP58) [NBP2-25221] -

AGR2 H117Y has a reduced ability to interact with MUC2 and regulate ER stress in vitro. (A) HEK293T cells were transfected with equal amounts of WT or H117Y mutant (Mut) HA-tagged AGR2 (HA-AGR2) and blotted for AGR2 or beta -actin as a loading control. Mutant HA-AGR2 consistently showed lower expression when examined by Western blot. Data are representative of 3 independent experiments. (B and right panel of C) Mutant AGR2 was transfected at double the amount compared with WT plasmid to obtain the same level, or higher, of mutant AGR2 protein as WT. (B) LS174T cells were transfected with WT or H117Y mutant HA-AGR2 or Green Fluorescent Protein (GFP) as a transfection control. Transfected AGR2 was immunoprecipitated using anti-HA beads, and immunoprecipitates were blotted for MUC2 (clone CCP58) and HA-tag. (C) Immunoblots of the lysates of HEK293T cells overexpressing WT or Mut AGR2 or GFP and stressed with tunicamycin (Tun) 48 hours after transfection. Left: Data from cells transfected with equal amounts of WT or Mut (1×) plasmid. Right: Results in which twice (2×) the amount of mutant AGR2 plasmid was transfected than for WT. Blots were stained with antibodies against BiP (clone C50B12), AGR2 (clone D9V2F), HA (clone C29F4), and beta -actin. Experiment was repeated 3 independent times in HEK293T. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34237462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MUC2 Antibody (CCP58)

Immunocytochemistry/ Immunofluorescence: MUC2 Antibody (CCP58) [NBP2-25221] -

(A) Western blot of full-length MUC2 protein (540 kDa) and beta -actin (43 kDa) in different cell lines LS-174T CTRL, LS-174T MUC2 K.O., HT-29 CTRL, and HT-29 MUC2 K.O. grown in 2D (N = 3 biological replicates). (B) Western blot of full-length MUC2 protein (540 kDa) and beta -actin (43 kDa) in different cell lines LS-174T CTRL, LS-174T MUC2 K.O., HT-29 CTRL, and HT-29 MUC2 K.O. grown in 2D (N = 3 biological replicates). (C) Representative images of spheroids of HT-29 and LS-174T CTRL and MUC2 K.O. immunostaining with mouse anti-hMUC2 antibody (green) and counterstained with DAPI. Images acquired on confocal LS780. Scale bar, 100 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39926604), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MUC2 Antibody (CCP58)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot reported in scientific literature (PMID: 27084834).

Reviewed Applications

Read 3 reviews rated 4.3 using NBP2-25221 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MUC2

MUC2, also called mucin 2, is a member of the mucin protein family which are heavily glycosylated, high molecular weight proteins that are produced by goblet cells in epithelial tissues including the intestinal and respiratory tract (1). Mucins can form gels and function in lubrication of mucosal surfaces which serve as a protective barrier of the epithelial lining and an innate host defense (1). Mucins are divided into two groups based on whether they are membrane-bound or secreted, where MUC2 belongs to the secretory group (1,2). Secreted mucins have a distinctive composition characterized by a large central exon "mucin domain" containing O-glycosylated tandem repeat regions rich in proline, threonine, and serine residues, non-repetitive domains, and cysteine-rich domains (1,2). The mucin domain is flanked by 5' and 3' D domains, a B-like domain, a C-like domain, and a Ck-domain (1,2). MUC2 was the first secretory mucin identified in humans (3,4). Full-length MUC2 protein is synthesized as 5179 amino acids (aa) in length with a theoretical molecular weight of ~540 kDa (5).

Changes or perturbations to MUC2 expression has been associated with a number of disease pathologies (1-4, 6). Specifically, altered MUC2 composition has been indicated in colorectal cancer, inflammatory bowel diseases (IBD) including ulcerative colitis and Chron's disease, and chronic obstructive pulmonary disease (COPD) (1-4, 6). In general, decreased or reduced MUC2 expression is associated with colorectal cancer disease progression and development of IBD (1-4, 6). Additionally, studies have found that upon intestinal infection due to parasites or bacteria MUC2 expression is increased, as is overall mucus secretion (3, 6).

References

1. Pothuraju, R., Krishn, S. R., Gautam, S. K., Pai, P., Ganguly, K., Chaudhary, S., Rachagani, S., Kaur, S., & Batra, S. K. (2020). Mechanistic and Functional Shades of Mucins and Associated Glycans in Colon Cancer. Cancers. https://doi.org/10.3390/cancers12030649

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of Clinical Medicine. https://doi.org/10.3390/jcm8091447

3. Liu, Y., Yu, X., Zhao, J., Zhang, H., Zhai, Q., & Chen, W. (2020). The role of MUC2 mucin in intestinal homeostasis and the impact of dietary components on MUC2 expression. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2020.07.191

4. Allen, A., Hutton, D. A., & Pearson, J. P. (1998). The MUC2 gene product: a human intestinal mucin. The International Journal of Biochemistry & Cell Biology. https://doi.org/10.1016/s1357-2725(98)00028-4

5. Uniprot (Q02817)

6. Kim, Y. S., & Ho, S. B. (2010). Intestinal goblet cells and mucins in health and disease: recent insights and progress. Current Gastroenterology Reports. https://doi.org/10.1007/s11894-010-0131-2

Alternate Names

Intestinal mucin-2, MLP, MUC2, MUC-2, mucin 2, intestinal/tracheal, mucin 2, oligomeric mucus/gel-forming, mucin-2, SMUC

Gene Symbol

MUC2

UniProt

NP_002448 (Human), Q02817 (Human)

Additional MUC2 Products

Product Documents for MUC2 Antibody (CCP58)

Certificate of Analysis

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Product Specific Notices for MUC2 Antibody (CCP58)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MUC2 Antibody (CCP58)

Customer Reviews for MUC2 Antibody (CCP58) (3)

4.3 out of 5
3 Customer Ratings
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Showing  1 - 3 of 3 reviews Showing All
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  • MUC2 Antibody (CCP58)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 04/04/2020
    MUC2 Antibody (CCP58) NBP2-25221
  • MUC2 Antibody (CCP58)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA MB 231 cells
    Species: Human
    Verified Customer | Posted 12/27/2019
    MUC2 Antibody (CCP58) NBP2-25221
  • MUC2 Antibody (CCP58)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA MB 231 cells and HCC827 human non-small cell lung cancer cell line
    Species: Human
    Verified Customer | Posted 10/17/2018
    MUC2 Antibody (CCP58) NBP2-25221

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