Muscle Phosphofructokinase/PFKM/PFK-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87293

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Muscle Phosphofructokinase/PFKM Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody - BSA Free

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293]

Immunocytochemistry/ Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293]

Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293]

Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293]

Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in human skeletal muscle tissue.

Applications for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Muscle Phosphofructokinase/PFKM

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Long Name

Phosphofructokinase, Muscle

Alternate Names

GSD7, PFK-1, PFK-A, PFKM, PFKX, Phosphofructokinase-1, Phosphofructokinase-M, Phosphohexokinase

Gene Symbol

PFKM

Additional Muscle Phosphofructokinase/PFKM Products

Product Documents for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

Citations for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody - BSA Free

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Protocols

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